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Piriformospora indica enhances the production of pentacyclic triterpenoids in Lantana camara L. suspension cultures

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Abstract

The stimulation of secondary metabolite synthesis by the endophytic fungus, Piriformospora indica, was investigated for the production of triterpenoids—ursolic acid (UA), oleanolic acid (OA) and betulinic acid (BA)—in the suspension cultures of Lantana camara. Growth and product formation profiles of the cell suspension cultures of L. camara were established for optimum elicitation strategy. The elicitation potential of P. indica was compared with typical elicitors like methyl jasmonate (MJ), chitin and chitosan. MJ at 62.5 µM and 2.5 % (v/v) filter-sterilized culture filtrate of P. indica enhanced the triterpenoids production to similar level. Filter-sterilized culture filtrate of P. indica at 2.5 % (v/v) increased the concentration of UA by 3.5-fold (3869.3 µg/g DW), OA by 5.6-fold (1425.7 µg/g DW) and BA by 7.8-fold (117.02 µg/g DW) in the cell cultures of L. camara.

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References

  • Adya AK, Gautam A, Zhang L, Varma A (2013) Characterization of Piriformospora indica culture filtrate. In: Varma A et al (eds) Piriformospora indica, soil biology, vol 33. Springer, Berlin, pp 345–375

    Chapter  Google Scholar 

  • Alakurtti S, Mäkelä T, Koskimies S, Yli-Kauhaluoma J (2006) Pharmacological properties of the ubiquitous natural product betulin. Eur J Pharm Sci 29:1–13

    Article  CAS  PubMed  Google Scholar 

  • Bagde US, Prasad R, Varma A (2011) Influence of culture filtrate of Piriformospora indica on growth and yield of seed oil in Helianthus annus. Symbiosis 53:83–88

    Article  Google Scholar 

  • Bagde US, Prasad R, Varma A (2013) Impact of culture fltrate of Piriformospora indica on biomass and biosynthesis of active ingredient aristolochic acid in Aristolochia elegans Mart. Int J Biol 6:29–37

    Article  Google Scholar 

  • Bahabadi SE, Sharifi M, Safaie N et al (2011) Increased lignan biosynthesis in the suspension cultures of Linum album by fungal extracts. Plant Biotechnol Rep 5:367–373

    Article  Google Scholar 

  • Baldi A, Jain A, Gupta N et al (2008) Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins: a first report. Biotechnol Lett 30:1671–1677

    Article  CAS  PubMed  Google Scholar 

  • Baldi A, Farkya S, Jain A et al (2010) Enhanced production of podophyllotoxins by co-culture of transformed Linum album cells with plant growth-promoting fungi. Pure Appl Chem 82:227–241

    Article  CAS  Google Scholar 

  • Das A, Kamal S, Shakil NA et al (2012) The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant, Coleus forskohlii. Plant Signal Behav 7:103–112

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gao FK, Dai CC, Liu XZ (2010) Mechanisms of fungal endophytes in plant protection against pathogens. African J Microbiol Res 4:1346–1351

    Google Scholar 

  • Ghisalberti EL (2000) Lantana camara L. (Verbenaceae). Fitoterapia 71:467–486

    Article  CAS  PubMed  Google Scholar 

  • Godoy-Hernández G, Vázquez-Flota FA (2012) Growth measurements: estimation of cell division and cell expansion. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Plant cell culture protocols, methods in molecular biology, vol 877. Humana Press, New York, pp 41–48

    Chapter  Google Scholar 

  • Kaefer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 131:19–33

    Google Scholar 

  • Kumar V, Sahai V, Bisaria VS (2013) Effect of Piriformospora indica on enhanced biosynthesis of anticancer drug, podophyllotoxin, in plant cell cultures of Linum album. In: Varma A et al (eds) Piriformospora indica, soil biology, vol 33. Springer, Berlin, pp 119–137

    Chapter  Google Scholar 

  • Kuźma Ł, Bruchajzer E, Wysokińska H (2009) Methyl jasmonate effect on diterpenoid accumulation in Salvia sclarea hairy root culture in shake flasks and sprinkle bioreactor. Enzyme Microb Technol 44:406–410

    Article  Google Scholar 

  • Liu J (2005) Oleanolic acid and ursolic acid: research perspectives. J Ethnopharmacol 100:92–94

    Article  CAS  PubMed  Google Scholar 

  • Rai M, Acharya D, Singh A, Varma A (2001) Positive growth responses of the medicinal plants Spilanthes calva and Withania somnifera to inoculation by Piriformospora indica in a field trial. Mycorrhiza 11:123–128

    Article  CAS  PubMed  Google Scholar 

  • Rai MK, Varma A, Pandey AK (2004) Antifungal potential of Spilanthes calva after inoculation of Piriformospora indica. Mycoses 47:479–481

    Article  CAS  PubMed  Google Scholar 

  • Rijhwani SK, Shanks JV (1998) Effect of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog 14:442–449

    Article  CAS  PubMed  Google Scholar 

  • Satheesan J, Narayanan AK, Sakunthala M (2012) Induction of root colonization by Piriformospora indica leads to enhanced asiaticoside production in Centella asiatica. Mycorrhiza 22:195–202

    Article  CAS  PubMed  Google Scholar 

  • Savitha BC, Thimmaraju R, Bhagyalakshmi N, Ravishankar GA (2006) Different biotic and abiotic elicitors influence betalain production in hairy root cultures of Beta vulgaris in shake-flask and bioreactor. Process Biochem 41:50–60

    Article  CAS  Google Scholar 

  • Sharma G, Agrawal V (2013) Marked enhancement in the artemisinin content and biomass productivity in Artemisia annua L. shoots co-cultivated with Piriformospora indica. World J Microbiol Biotechnol 29:1133–1138

    Article  CAS  PubMed  Google Scholar 

  • Singh A, Singh A, Kumari M, Rai MK, Varma A (2003) Biotechnology importance of Piriformospora indica—a novel symbiotic mycorrhiza-like fungus: an overview. Indian J Biotechnol 2:65–75

    Google Scholar 

  • Singh A, Rajpal K, Singh M, Kharkwal AC, Arora M, Varma A (2013) Mass cultivation of Piriformospora indica and Sebacina species. In: Varma A et al (eds) Piriformospora indica, soil biology, vol 33. Springer, Berlin, pp 377–392

    Chapter  Google Scholar 

  • Sirrenberg A, Göbel C, Grond S et al (2007) Piriformospora indica affects plant growth by auxin production. Physiol Plant 131:581–589

    Article  CAS  PubMed  Google Scholar 

  • Srivastava P, Chaturvedi R (2010) Simultaneous determination and quantification of three pentacyclic triterpenoids—betulinic acid, oleanolic acid, and ursolic acid—in cell cultures of Lantana camara L. In Vitro Cell Dev Biol Plant 46:549–557

    Article  CAS  Google Scholar 

  • Srivastava P, Kasoju N, Bora U, Chaturvedi R (2010) Accumulation of betulinic, oleanolic, and ursolic acids in in vitro cell cultures of Lantana camara L. and their significant cytotoxic effects on HeLa cell lines. Biotechnol Bioprocess Eng 15:1038–1046

    Article  CAS  Google Scholar 

  • Srivastava P, Sisodia V, Chaturvedi R (2011) Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioprocess Biosyst Eng 34:75–80

    Article  CAS  PubMed  Google Scholar 

  • Varma A, Savita V, Sudha et al (1999) Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Appl Environ Microbiol 65:2741–2744

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wiktorowska E, Długosz M, Janiszowska W (2010) Significant enhancement of oleanolic acid accumulation by biotic elicitors in cell suspension cultures of Calendula officinalis L. Enzyme Microb Technol 46:14–20

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Peeyush Kumar, Durai Sundar & V. S. Bisaria

  2. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Rakhi Chaturvedi

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  1. Peeyush Kumar
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  2. Rakhi Chaturvedi
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  3. Durai Sundar
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  4. V. S. Bisaria
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Correspondence to V. S. Bisaria.

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Kumar, P., Chaturvedi, R., Sundar, D. et al. Piriformospora indica enhances the production of pentacyclic triterpenoids in Lantana camara L. suspension cultures. Plant Cell Tiss Organ Cult 125, 23–29 (2016). https://doi.org/10.1007/s11240-015-0924-y

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  • DOI: https://doi.org/10.1007/s11240-015-0924-y

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