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In vitro organogenesis of Cedrela fissilis Vell. (Meliaceae): the involvement of endogenous polyamines and carbohydrates on shoot development

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Abstract

Cytokinins are essential molecules involved in shoot induction during in vitro propagation. The addition of cytokinins into culture medium may modulate the endogenous metabolism of various compounds that play important roles during in vitro organogenesis responses, such as carbohydrates and polyamines (PAs). The effects of 6-benzyladenine (BA) and the type of explant (apical and cotyledonary nodal segments) on shoot development and the endogenous content of carbohydrates and free PAs were analyzed during the in vitro culture of Cedrela fissilis. BA addition (2.5 µM) into the culture medium was essential to increase both the number of shoots arising from the apical nodal segments and the length of the shoots arising from the cotyledonary nodal segments. Increased endogenous glucose was associated with an increased shoot length in the shoots derived from the cotyledonary nodal segments cultured with BA. On the other hand, increased endogenous sucrose was associated with reduced shoot production by apical nodal segments cultured without BA. Supplementation of the culture medium with BA increased the endogenous content of free putrescine in the apical nodal segments and increased the number of shoots produced compared to nodes cultured without BA. Total free PAs was increased in those treatments that increased the number of shoots produced. This work is the first to report the relationship between the addition of BA to the culture medium and the endogenous contents of soluble carbohydrates and free PAs during the in vitro propagation of tree species.

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Abbreviations

ANOVA:

Analysis of variance

BA:

6-Benzyladenine

FM:

Fresh matter

HPLC:

High performance liquid chromatography

PAs:

Polyamine

MS:

Murashige and Skoog

PGRs:

Plant growth regulators

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

Funding for this work was provided by the National Council for Scientific and Technological Development (CNPq) (476465/2011-7 and 305645/2013-7) and Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ) (E26/112.055/2011, E26/110.390/2012, E26/111.389-2012, E26/102.989/2012, E26/010.001507/2014). VPMA thanks Coordination for the Improvement of Higher Education Personnel (CAPES), and YRSR thanks CNPq for the fellowship.

Author contribution

C. S. C., V. S. and V. P. M. A. conceived the study, designed the experiments and wrote the manuscript. V. P. M. A. and Y. R. S. R. were responsible for the in vitro culture data. V. P. M. A. analyzed the carbohydrates and PAs and performed the statistical analyses. R. S. R. collaborated on the carbohydrate analysis by HPLC. E. I. S. F. and A. F. M. were responsible for the analysis of PAs. All of the authors read and approved the final manuscript.

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Authors and Affiliations

  1. Laboratório de Biologia Celular e Tecidual (LBCT)-CBB, Universidade Estadual do Norte Fluminense “Darcy Ribeiro”, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ, CEP 28013-602, Brazil

    Victor Paulo Mesquita Aragão, Yrexam Rodrigues de Souza Ribeiro & Claudete Santa-Catarina

  2. Laboratório de Biotecnologia (LBT)-CBB, Universidade Estadual do Norte Fluminense “Darcy Ribeiro”, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis & Vanildo Silveira

  3. Laboratório de Biologia Celular de Plantas, Departamento de Botânica, IB-Universidade de São Paulo, São Paulo, Brazil

    Amanda Ferreira Macedo & Eny Iochevet Segal Floh

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  1. Victor Paulo Mesquita Aragão
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  2. Yrexam Rodrigues de Souza Ribeiro
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  3. Ricardo Souza Reis
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Correspondence to Claudete Santa-Catarina.

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Aragão, V.P.M., de Souza Ribeiro, Y.R., Reis, R.S. et al. In vitro organogenesis of Cedrela fissilis Vell. (Meliaceae): the involvement of endogenous polyamines and carbohydrates on shoot development. Plant Cell Tiss Organ Cult 124, 611–620 (2016). https://doi.org/10.1007/s11240-015-0919-8

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