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Antiporter NHX2 differentially induced in Mesembryanthemum crystallinum natural genetic variant under salt stress

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Abstract

Plants exhibit several mechanisms to survive under high salinity conditions. The uptake and compartmentalization of Na+ ion by the NHX antiporter is a crucial mechanism in homeostasis maintenance. Therefore, we evaluated McNHX2 gene expression and several physiological responses induced in three natural genetic variants of ice plants under salt stress. Based on morphology and growth behavior of wild type populations from an arid region of northwestern Mexico, we identified three ice plant natural genetic variants and called P0, P9, and P11. Several physiological parameters, such as water potential, relative water content, chlorophyll, and Na+ and K+ ion contents from all natural genetic variants exhibited a differential response under high salinity conditions. Specifically, the P0 variant showed lower water potential changes and least perturbation of Na+/K+ ratio than those of the P9 and P11 variants under saline conditions, suggesting that the P0 variant is the most salt tolerant. Unexpectedly, McNHX2 expression was repressed in the P11 variant while it was upregulated in the P0 and P9 variants under saline treatments. The McNHX2 gene was sequenced showing 15 introns and 16 exons; polymorphisms were found among the cDNAs sequences from the three natural genetic variants. All these data suggest that differential responses to high salinity involve different mechanisms operating in each variant for counteracting saline stress effects.

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Acknowledgments

Special thanks to Julio Hernández González and Carlos Cabada Tavares for technical support and Diana Dorantes for editorial services. This work was supported by the National Council of Science and Technology (CONACYT) Grant 156563 and 118866.

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  1. Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingenierías, Universidad Autónoma de Yucatán (UADY), Periférico Norte Kilómetro 33.5, Tablaje Catastral 13615 Chuburná de Hidalgo Inn, CP 97203, Mérida, Yucatán, Mexico

    Claudia Villicaña

  2. Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Avenida Instituto Politécnico Nacional No. 195, Col. Playa Palo de Santa Rita, P.O. Box 128, CP 23096, La Paz, B.C.S., Mexico

    Norberto Warner, Mario Arce-Montoya, Mario Rojas, Carlos Angulo & Gracia Gómez-Anduro

  3. Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Campus Guerrero Negro. Independencia y Paseo Eucalipto s/n, CP 23940, Guerrero Negro, B.C.S., Mexico

    Andrés Orduño

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  1. Claudia Villicaña
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Villicaña, C., Warner, N., Arce-Montoya, M. et al. Antiporter NHX2 differentially induced in Mesembryanthemum crystallinum natural genetic variant under salt stress. Plant Cell Tiss Organ Cult 124, 361–375 (2016). https://doi.org/10.1007/s11240-015-0900-6

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