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Salinity-induced metabolic profile changes in Nitraria tangutorum Bobr. suspension cells

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Abstract

Salinity is an important abiotic stress that severely depresses plant growth and development. Plants have evolved salinity tolerance mechanisms to enable adaptation to hostile environments. Generally, metabolic networks must be reconfigured to maintain metabolic homeostasis and to mitigate the stress. However, the mechanisms of plant stress-response systems are incompletely understood, which represents a bottleneck for further use of salinity-tolerant plants. Through its capability to provide both an overview and detailed analysis of changes in metabolic pathways, metabolomics has been widely applied to research plants’ stress resistance. In this study a metabolomics approach was adopted to describe changes in the metabolic profile of Nitraria tangutorum Bobr. suspension cells under salinity stress. Four salinity treatments (100, 150, 200, and 250 mM NaCl) were applied and liquid medium lacking NaCl was used as the control. After culture for 4 days, salinity stress affected the biomass, cell morphology, and osmotic potential of the suspension cells. Totals of 373 and 629 peaks were detected by gas chromatography/time-of-flight mass spectrometry in the aqueous phase and organic phase, respectively. Metabolic profiling differences were visualized by partial least squares-discriminant analysis models to highlight the changes in metabolic pathways under salinity stress. Twenty-six metabolites that showed differences in content were identified, including sugars, amino acids, and fatty acids. This research provides novel insights into the complex of metabolic pathways involved in the synthesis of stress-related metabolites, and of the salinity-tolerance mechanism in N. tangutorum suspension cells.

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Abbreviations

CE:

Capillary electrophoresis

DW:

Dried weight

FTIR:

Fourier transform infrared

FW:

Fresh weight

GC:

Gas chromatography

LC:

Liquid chromatography

MS:

Murashige and Skoog

NMR:

Nuclear magnetic resonance

PLS–DA:

Partial least squares-discriminant analysis

RGR:

Relative growth rate

SIMCA:

Soft independent modeling of class analogy

TCA:

Tricarboxylic acid cycle

VIP:

Variable importance in the projection

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Acknowledgments

This work is financially supported by the Special Funds Projects for Basic Scientific Research Business Expenses of Central Public Welfare Research Institutes in Chinese Academy of Forestry (Grant No. CAFYBB2012009; No. CAFYBB2011005-5).

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Authors and Affiliations

  1. Research Center of Saline and Alkali Land of State Forestry Administration, Chinese Academy of Forestry, Beijing, 100091, China

    Jianwei Ni, Xiuyan Yang, Jianfeng Zhu, Zhengxiang Liu, Haiwen Wu, Huaxin Zhang & Tao Liu

  2. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China

    Jianwei Ni

  3. Engineering and Technology Research for Fruits and Vegetables Processing, China Agricultural University, Beijing, 100083, China

    Yuanying Ni

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  1. Jianwei Ni
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  2. Xiuyan Yang
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  3. Jianfeng Zhu
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Correspondence to Huaxin Zhang.

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Ni, J., Yang, X., Zhu, J. et al. Salinity-induced metabolic profile changes in Nitraria tangutorum Bobr. suspension cells. Plant Cell Tiss Organ Cult 122, 239–248 (2015). https://doi.org/10.1007/s11240-015-0744-0

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