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Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate

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Abstract

Establishment of a stable and efficient in vitro regeneration system from leaf explants is a basic requirement for success in genetic transformation of peach. In this study, approximately 900 leaf explants of ‘Guardian®’ peach genotype were separated from in vitro shoots and cultured for shoot regeneration on ½ MS medium with different doses and combinations of thidiazuron (TDZ) and silver thiosulphate (STS). The results showed that callus bud formation and shoot regeneration occurred 4 and 6 weeks after culture initiation, respectively. All the regenerated shoots were from petioles of the leaf explants. Regeneration rates were significantly affected by different combinations of TDZ and STS (P < 0.05). Separation of means by Tukey test method revealed that on half strength MS media, high regeneration rates were obtained from the combinations of 6.81 µM TDZ + 10 µM STS, and of 9.08 µM TDZ + 10 µM STS (16 and 14 %, respectively). The number of shoots per regenerating explants was also significantly affected by growth regulator concentrations. The highest shoot number was obtained with the combination of 6.81 µM TDZ + 10 µM STS. Rooting percentage varied from 85 to 100 % depending on plant growth regulator used. On half strength MS medium containing 1.0 mg/l of IBA, an average of 7 roots per regenerating shoot was produced. The rooted plantlets were successfully acclimatized with 92 % survival rate under greenhouse conditions. This established regeneration system from leaf explants would facilitate the development of reliable peach genetic transformation procedures, helping accelerate peach genetic improvement using biotechnology approaches.

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Abbreviations

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

IBA:

Indole butyric acid

MS:

Murashige and Skoog medium

NAA:

Naphthalene acetic acid

STS:

Silver thiosulfate

TDZ:

Thidiazuron

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Acknowledgments

This work was supported by South Carolina Peach Council, and the U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service (Grant Nos. SC1700315 and SC-1700450). Bekir San was supported by a fellowship from the Scientific and Technological Research Council of Turkey (TUBITAK). This is Technical Contribution No. 6294 of the Clemson University Experiment Station.

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Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, Suleyman Demirel University, 32260, Isparta, Turkey

    Bekir San

  2. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Bekir San, Zhigang Li, Qian Hu & Hong Luo

  3. School of Agricultural, Forest, and Environmental Sciences, Clemson University, Clemson, SC, 29634, USA

    Gregory L. Reighard

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  1. Bekir San
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  2. Zhigang Li
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  3. Qian Hu
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  4. Gregory L. Reighard
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  5. Hong Luo
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Correspondence to Hong Luo.

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San, B., Li, Z., Hu, Q. et al. Adventitious shoot regeneration from in vitro cultured leaf explants of peach rootstock Guardian® is significantly enhanced by silver thiosulfate. Plant Cell Tiss Organ Cult 120, 757–765 (2015). https://doi.org/10.1007/s11240-014-0645-7

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  • DOI: https://doi.org/10.1007/s11240-014-0645-7

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