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Genetic fidelity of long-term micropropagated Lavandula officinalis Chaix.: an important aromatic medicinal plant

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Abstract

A protocol for long-term in vitro conservation of multiple shoot cultures of Lavandula officinalis and regeneration of true-to-type plants from them is described here. Multiple shoots were developed from apical bud explants on a modified vitamin-enriched Murashige and Skoog’s basal medium supplemented with 2.5 mg/l Kinetin. The cultures were subsequently conserved in vitro for 6 years under slow growth conditions imposed by lowering the sucrose level from 3 to 2.0 % and increasing the agar concentration from 0.8 to 1.2 % in this medium. Complete plant regeneration from in vitro conserved shoots was achieved through axillary shoot proliferation on original 2.5 mg/l Kinetin containing medium followed by rooting of individual shoots in a hormone-free half strength MS basal medium. The genetic fidelity of the regenerated plants was tested by random amplified polymorphic DNA analysis. Twenty one arbitrary decamer primers produced a total of 64 scorable bands (1–6 bands/primer) in the size range of 100–5,148 bp. All DNA fingerprints with these primers displayed monomorphic band profiles indicating homogeneity among the regenerated progeny and their uniformity with the donor parent. Head-space gas chromatographic analysis of the leaf tissue excised from mother as well as regenerated plants also revealed a similar qualitative and quantitative profile of volatile terpenes. The utility of the developed protocol for long-term ex situ conservation and quality plant production for cultivation of this high value aromatic herb is discussed.

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Acknowledgments

The authors express gratitude to The Director, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow for the facilities, and Department of Biotechnology (DBT), Government of India, New Delhi for the financial assistance. A part of this work was also carried out under a CSIR network project (ChemBio-BSC 0203). The first author (AP) also thanks Council of Scientific & Industrial Research, New Delhi for awarding a Senior Research Fellowship to her during this investigation.

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Authors and Affiliations

  1. Plant Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Lucknow, 226015, India

    Archana Prasad, Shastri Prasad Shukla, Archana Mathur & Ajay Kumar Mathur

  2. Laboratory of Aromatic Plants and Chiral Separation, CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Council of Scientific and Industrial Research, PO CIMAP, Lucknow, 226015, India

    Chandan Singh Chanotiya

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  1. Archana Prasad
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  2. Shastri Prasad Shukla
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  3. Archana Mathur
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  4. Chandan Singh Chanotiya
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  5. Ajay Kumar Mathur
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Correspondence to Ajay Kumar Mathur.

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Prasad, A., Shukla, S.P., Mathur, A. et al. Genetic fidelity of long-term micropropagated Lavandula officinalis Chaix.: an important aromatic medicinal plant. Plant Cell Tiss Organ Cult 120, 803–811 (2015). https://doi.org/10.1007/s11240-014-0637-7

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  • DOI: https://doi.org/10.1007/s11240-014-0637-7

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