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Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures

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Abstract

We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15–24 days. Methanolic extracts of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants. Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) medium

TDZ:

Thidiazuron

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Acknowledgments

This work was supported by the Thailand Research Fund (DBG4980009). SK is grateful for financial support from the Royal Golden Jubilee Program (PHD/0143/2548).

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand

    Panitch Boonsnongcheep, Sirintra Korsangruang, Promchit Saralamp & Sompop Prathanturarug

  2. Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand

    Noppamas Soonthornchareonnon

  3. Department of Botany, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand

    Yupyn Chintapakorn

Authors
  1. Panitch Boonsnongcheep
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  2. Sirintra Korsangruang
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  3. Noppamas Soonthornchareonnon
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  4. Yupyn Chintapakorn
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  5. Promchit Saralamp
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  6. Sompop Prathanturarug
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Correspondence to Sompop Prathanturarug.

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Boonsnongcheep, P., Korsangruang, S., Soonthornchareonnon, N. et al. Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tiss Organ Cult 101, 119–126 (2010). https://doi.org/10.1007/s11240-010-9668-x

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  • DOI: https://doi.org/10.1007/s11240-010-9668-x

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