Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 99, Issue 1, pp 27–34

Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa

Authors

    • Institute of Ornamental and Woody Plant ScienceLeibniz University Hannover
  • M. Serek
    • Institute of Ornamental and Woody Plant ScienceLeibniz University Hannover
  • T. Winkelmann
    • Institute of Ornamental and Woody Plant ScienceLeibniz University Hannover
Original Paper

DOI: 10.1007/s11240-009-9572-4

Cite this article as:
Meyer, L., Serek, M. & Winkelmann, T. Plant Cell Tiss Organ Cult (2009) 99: 27. doi:10.1007/s11240-009-9572-4

Abstract

Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

Keywords

Protoplast yield Callus formation Plant regeneration and stability

Abbreviations

BA

6-Benzyladenine

FW

Fresh weight

IAA

Indole-3-acetic acid

MS

Murashige and Skoog (1962)

NAA

1-Naphthaleneacetic acid

TDZ

Thidiazuron

Copyright information

© Springer Science+Business Media B.V. 2009