Plant Cell, Tissue and Organ Culture

, 95:13

Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis

  • Claudia Luna
  • Mónica Collavino
  • Luis Mroginski
  • Pedro Sansberro
Original Paper

DOI: 10.1007/s11240-008-9408-7

Cite this article as:
Luna, C., Collavino, M., Mroginski, L. et al. Plant Cell Tiss Organ Cult (2008) 95: 13. doi:10.1007/s11240-008-9408-7

Abstract

Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM™ were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml−1 cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth.

Keywords

Micropropagation of woody plantsContaminationRITA®16S rDNA analysisCefotaxime

Abbreviations

Ap

Ampicilline

Cf

Cefotaxime

Cm

Chloramphenicol

Gm

Gentamycin

Km

Kanamycin

Sm

Streptomycin

PPM™

Plant preservative mixture™

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Claudia Luna
    • 1
  • Mónica Collavino
    • 1
  • Luis Mroginski
    • 1
  • Pedro Sansberro
    • 1
  1. 1.Facultad de Ciencias Agrarias (UNNE)Instituto de Botánica del NordesteCorrientesArgentina