Original Paper

Plant Cell, Tissue and Organ Culture

, Volume 95, Issue 1, pp 13-19

Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis

  • Claudia LunaAffiliated withFacultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste
  • , Mónica CollavinoAffiliated withFacultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste
  • , Luis MroginskiAffiliated withFacultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste
  • , Pedro SansberroAffiliated withFacultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste Email author 

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Abstract

Endogenous bacterial contaminants isolated from infected cultures of Ilex dumosa nodal segments were identified as Stenotrophomonas maltophilia and Achromobacter sp. using 16S rDNA analysis. A range of antibiotics with different mechanism of actions and the commercial biocide PPM™ were tested for their capacity to repress the growth of Gram negative bacteria grown in liquid medium during the establishment phase of temporal immersion systems. The best results were obtained with the addition of 0.5 mg ml−1 cefotaxime to the culture media obtaining 100% of uncontaminated cultures without suppress of shoot growth.

Keywords

Micropropagation of woody plants Contamination RITA® 16S rDNA analysis Cefotaxime