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Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet

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Abstract

Both biotic and abiotic stresses cause considerable crop yield losses worldwide (Chrispeels, Sadava Plants, genes, and crop biotechnology 2003; Oerke, Dehne Crop Prot 23:275–285 2004). To speed up screening assays in stress resistance breeding, non-contact techniques such as chlorophyll fluorescence imaging can be advantageously used in the quantification of stress-inflicted damage. In comparison with visual spectrum images, chlorophyll fluorescence imaging reveals cell death with higher contrast and at earlier time-points. This technique has the potential to automatically quantify stress-inflicted damage during screening applications. From a physiological viewpoint, screening stress-responses using attached plant leaves is the ideal approach. However, leaf growth and circadian movements interfere with time-lapse monitoring of leaves, making it necessary to fix the leaves to be studied. From this viewpoint, a method to visualise the evolution of chlorophyll fluorescence from excised leaf pieces kept in closed petri dishes offers clear advantages. In this study, the plant–fungus interaction sugar beet–Cercospora beticola was assessed both in attached leaf and excised leaf strip assays. The attached leaf assay proved to be superior in revealing early, pre-visual symptoms and to better discriminate between the lines with different susceptibility to Cercospora.

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Abbreviations

BA:

6-Benzylaminopurine

Chl-FI:

Chlorophyll fluorescence imaging

dpi:

Days postinfection

FL :

Chlorophyll fluorescence image captured after low intensity excitation

FH :

Chlorophyll fluorescence image captured after high intensity excitation

FIS:

Fluorescence imaging system

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Acknowledgements

L.C. is a post-doctoral fellow of the Research Foundation—Flanders. D.H. is a post-doc with financial support provided through the European Community’s Human Potential Programme under contract HPRN-CT-2002–00254, STRESSIMAGING. The authors are grateful to Roland Valcke, Laboratory for Molecular and Physical Plant Physiology, Hasselt University, for advice on chlorophyll fluorescence imaging.

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Authors and Affiliations

  1. Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, Gent, 9000, Belgium

    Laury Chaerle, Dik Hagenbeek & Dominique Van Der Straeten

  2. Phytopathology Department, SESVanderHave, Soldatenplein Z2, Industriepark 15, Tienen, 3300, Belgium

    Erik De Bruyne

Authors
  1. Laury Chaerle
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  2. Dik Hagenbeek
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  3. Erik De Bruyne
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  4. Dominique Van Der Straeten
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Correspondence to Laury Chaerle or Dominique Van Der Straeten.

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Chaerle, L., Hagenbeek, D., De Bruyne, E. et al. Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet. Plant Cell Tiss Organ Cult 91, 97–106 (2007). https://doi.org/10.1007/s11240-007-9282-8

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  • DOI: https://doi.org/10.1007/s11240-007-9282-8

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