REVIEW

Plant Cell, Tissue and Organ Culture

, Volume 86, Issue 3, pp 303-318

Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants

  • Marek HraškaAffiliated withFaculty of Biological Sciences, Department of Genetics, University of South BohemiaFaculty of Agriculture, Biotechnological Centre, University of South Bohemia
  • , Slavomír RakouskýAffiliated withFaculty of Biological Sciences, Department of Genetics, University of South BohemiaFaculty of Health and Social Studies, University of South Bohemia Email author 
  • , Vladislav ČurnAffiliated withFaculty of Agriculture, Biotechnological Centre, University of South Bohemia

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Transformation of plants is a popular tool for modifying various desirable traits. Marker genes, like those encoding for bacterial β-glucuronidase (GUS), firefly luciferase (LUC) or jellyfish green fluorescent protein (GFP) have been shown to be very useful for establishing of efficient transformation protocols. Due to favourable properties such as no need of exogenous substrates and easy visualization, GFP has been found to be superior in to other markers in many cases. However, the use of GFP fluorescence is associated with some obstacles, mostly related to the diminishing of green fluorescence in older tissues, variation in fluorescence levels among different tissues and organs, and occasional interference with other fluorescing compounds in plants. This paper briefly summarizes basic GFP properties and applications, and describes in more detail the contribution of GFP to the establishment, evaluation and improvement of transformation procedures for plants. Moreover, features and possible obstacles associated with monitoring GFP fluorescence are discussed.

Keywords

Agrobacterium tumefaciens Green fluorescent protein Particle bombardment Plant transformation Selection