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Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

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Abstract

Factors affecting successful establishment in vitro, rapid proliferation and rooting of apricot cultivar ‘Bebecou’ were studied. Ethanol and NaOCl were applied in several combinations for disinfection; chilling, plant growth regulators BA, IAA and GA3, antibiotics, different culture vessels and systems of subculture were evaluated for the optimization of shoot proliferation and the auxins NAA and IBA were assessed for root induction. The highest number of new microshoots/explant (18.7) was obtained in a culture medium supplemented with 2.2 μM BA+0.57 μM IAA after 300 h of chilling. The effect of GA3 (11.4 μM) on shoot proliferation was positive in combination with 4.4 or 8.9 μM BA. Shoot length and productivity were highest at 2.2 μM BA+11.4 μM GA3+0.57 μM IAA and at 2.2 μM BA+0.57 μM IAA, respectively and decreased as cytokinin concentration increased. The antibiotic ‘Na-cefotaxime’ had a minimal impact on shoot growth when used at the lowest concentration (250 mg l−1). Subculture every 2 weeks in a medium supplemented with 2.2 μM BA and 0.57 μM IAA was more efficient for shoot induction than alternation of 20 days culture in a propagation medium supplemented with 2.2 μM BA and 10 days culture in an elongation medium supplemented with 1.1 μM BA and 5.71 μM IAA. The highest number of roots/shoot (8.1) was recorded at 19.6 μM IBA.

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Abbreviations

BA:

6-benzyladenine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

NAA:

α-naphthaleneacetic acid

References

  • Al-Maarri K, Arnaud Y, Miginiac E, (1994) Micropropagation of Pyrus communis cultivar Passe Crassane seedlings and cultivar Williams: factors affecting root formation in vitro and ex vitroSci. Hortic. 58: 207–214

    Article  Google Scholar 

  • Balla I, Vertesy J, (2001) In vitro culture of Hungarian apricot varietiesActa Hortic. 560: 395–398

    CAS  Google Scholar 

  • Bouza L, Jacques M, Maziere Y, Arnaud Y, (1992) In vitro propagation of Prunus tenella Batsch cv. Firehill – Control of vitrification – Increase of the multiplication rate and growth by chillingSci. Hortic. 52: 143–155

    Article  Google Scholar 

  • Brooks HJ, Hough LF, (1958) Vernalization studies with peach embryosProc. Am. Soc. Hort. Sci. 71: 95–102

    CAS  Google Scholar 

  • Campbell R, (1985) Plant Microbiology, Arnold, London, p. 191

  • Cassells AC, (2001) Contamination and its impact on tissue cultureActa Hortic. 560: 353–359

    Google Scholar 

  • Harada H, Murai Y, (1996) Micropropagation of Prunus mumePlant Cell Tiss. Org. Cult. 46: 265–267

    Article  CAS  Google Scholar 

  • Kataewa NV, Kramarenko MA, (1989) Clonal micropropagation of apricotByulleten Glavnogo Botanichekogo Sada 153: 69–73

    Google Scholar 

  • Kramarenko L, (1999) Micropropagation of apricot and field performance of in vitro propagated plantsActa Hortic. 488: 417–420

    Google Scholar 

  • Kratz KA, Langhans RW, (1978) Tissue culture propagation and dormancy studies on Clematis cultivarsHortScience 13: 356–357

    Google Scholar 

  • Lauri P, Caboni E, Damiano C, (2001) In vitro adventitious shoot regeneration from vegetative apices of almond and other Prunus speciesActa Hortic. 560: 403–406

    CAS  Google Scholar 

  • Marino G, Bertazza G, Magnanini E, Doro Altan A, (1993) Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricotPlant Cell, Tiss. Org. Cult. 34, 235–244

    Article  CAS  Google Scholar 

  • Murai Y, Harada H, Yamashita H, (1997) In vitro propagation of apricot (Prunus armeniaca L.) cv. ‘Bakuoh junkyou’J. Jpn. Soc. Hortic. Sci. 66: 475–480

    Article  CAS  Google Scholar 

  • Perez-Tornero O, Burgos L, Egea J, (1999) Introduction and establishment of apricot in vitro through the regeneration of shoots from meristem tipsIn Vitro Cell. Dev. Biol. –Plant 35: 249–253

    Article  Google Scholar 

  • Perez-Tornero O, Lopez JM, Egea J, Burgos L, (2000) Effect of basal media and growth regulators on the in vitro propagation of the apricot cultivar ‘Canino’J. Hort. Sci. Biotechnol. 75: 283–286

    CAS  Google Scholar 

  • Perez-Tornero O, Burgos L, (2000) Different media requirements for micropropagation of apricot cultivarsPlant Cell Tiss. Org. Cult. 63: 133–141

    Article  Google Scholar 

  • Poulsen GB, (1988) Elimination of contaminating micro-organisms from meristem culture of apple rootstock M26Acta Hortic. 225: 193–197

    Google Scholar 

  • Skirvin RM, Chu MC, Rukan H, (1979) Tissue culture of peach, sweet and sour cherry and apricot shoot tips. Proc. 124th Ann. Meeting Illi. State Hort. Soc: 30–38

  • Snir I, (1984) In vitro propagation of ‘Canino’ apricotHortScience 19: 229–230

    Google Scholar 

  • Valles M, Boxus Ph, (1987). Micropropagation of several Rosa hybrida L. cultivarsActa Hortic. 212: 611–617

    Google Scholar 

  • Vasilakakis M, Koukouryannis V, (1999) Apricot production in GreeceActa Hortic. 488: 43–50

    Google Scholar 

  • Welander M, (1983) In vitro rooting of the apple rootstock M 26 in adult juvenile and growth phases and acclimatization of the plantletsPhysiol. Plant. 58: 231–238

    Article  CAS  Google Scholar 

  • Yepes LM, Aldwinckle HS, (1994) Micropropagation of thirteen Malus cultivars and rootstocks and effect of antibiotics on proliferationPlant Growth Regul. 15: 55–67

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Pomology Lab, School of Agriculture, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece

    Georgios Koubouris & Miltiadis Vasilakakis

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  1. Georgios Koubouris
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  2. Miltiadis Vasilakakis
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Correspondence to Georgios Koubouris.

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Koubouris, G., Vasilakakis, M. Improvement of in vitro propagation of apricot cultivar ‘Bebecou’. Plant Cell Tiss Organ Cult 85, 173–180 (2006). https://doi.org/10.1007/s11240-005-9066-y

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  • DOI: https://doi.org/10.1007/s11240-005-9066-y

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