Research note

Plant Cell, Tissue and Organ Culture

, Volume 85, Issue 1, pp 115-121

First online:

Variation in leaf structures of micropropagated rhubarb (Rheum rhaponticum L.) PC49

  • Yipeng ZhaoAffiliated withHorticultural Department, Henan Institute of Science and Technology, Email author 
  • , Yan ZhouAffiliated withDepartment of Plant Science and Technology, Henan Institute of Science and Technology
  • , Brian W.W. GroutAffiliated withPostgraduate School, Writtle College,

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Alterations in leaf trichomes, stomatal characteristics and epidermal cellular features were investigated in micropropagated rhubarb (Rheum rhaponticum L.) PC49. The results showed that micropropagated regenerants had produced significantly lower stomatal index, but larger epidermal cell size than conventional plants. In addition, altered trichome morphology and abnormal stomata, e.g. twin-stomata were constantly noted only in micropropagated plants. The microscopic observation demonstrated a substantially larger intercellular space in palisade and mesophyll only in leaves of micropropagated plants. But the results showed no difference in chloroplast number and chlorophyll content between micropropagated and conventional plants. All the abnormalities suggest somaclonal variation may have occurred in micropropagated rhubarb PC49.


cellular structure somaclonal variation trichome twin-stomata