Confocal Raman Spectroscopy of Biological Objects under Conditions of Photoinduced Luminescence Self-Quenching
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- Naumenko, D., Snopok, B.A., Serviene, E. et al. Theor Exp Chem (2013) 49: 228. doi:10.1007/s11237-013-9320-z
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A study was carried out on the mechanism of luminescence self-quenching of heterogeneous biological objects using laser irradiation at 532 nm (50 μW/ μm2) in the format of a confocal microscope. Laser irradiation was found to reduce the luminescence intensity of material by a factor of 3–5, which permits us to obtain informative Raman scattering spectra of the dried cellular envelope of yeast. For irradiation times less than ~3-5 min, quenching is probably due either to formation of radiationless complexes of the excited chromophore molecules with the environment (excimers and/or exciplexes) or partial photodecomposition. Longer irradiation times lead to thermal degradation of the samples with the appearance of peaks corresponding to amorphous carbon.