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The role of the Robertsonian rearrangements in the origin of the XX/XY1Y2 sex chromosome system and in the chromosomal differentiation in Harttia species (Siluriformes, Loricariidae)

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Abstract

Harttia is a genus of the subfamily Loricariinae that posses a broad chromosomal variation. In addition to interspecific karyotype diversity within this group, a multiple sex chromosome system, XX/XY1Y2, has been described for Harttia carvalhoi. Thus, this study aimed to determine the role of chromosomal rearrangements in karyotype differentiation in Harttia by classical and molecular cytogenetic procedures. The results show that Robertsonian rearrangements have a prominent role in the chromosomal diversification of the species analysed, which initially leads to hypothesize a diploid number reduction in Harttia torrenticola and H. carvalhoi. The metacentric chromosome 1, shared between H. torrenticola and H. carvalhoi, could have originated from centric fusions from the ancestral karyotype. A centric fission event associated with the first metacentric pair allowed for the origination of a multiple sex chromosome system XX/XY1Y2, specific to H. carvalhoi. This study highlights the relevance of Robertsonian rearrangements in karyotypic differentiation of the species studied and demonstrates that the occurrence of a centric fission, as opposed to a previously hypothesised chromosome fusion, is directly implicated in the origin of the sex chromosome system of H. carvalhoi.

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Acknowledgments

The authors are grateful to Osvaldo Takeshi Oyakawa (MZUSP) for the identification of the specimens; to Luis Henrique da Silva and Pedro Luis Gallo for help with the sampling; to the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA—License number: 10538-1) and the Instituto Estadual de Florestas (IEF/MG) for authorization to collect the material. This study was financed by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná).

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Authors and Affiliations

  1. Departamento de Genética e Evolução, Universidade Federal de São Carlos, Rodovia Washington Luís (SP 310) Km 235, São Carlos, SP, 13565-905, Brazil

    Daniel Rodrigues Blanco, Roberto Laridondo Lui, Luiz Antônio Carlos Bertollo, Josiane Baccarin Traldi & Orlando Moreira-Filho

  2. Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil

    Marcelo Ricardo Vicari

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  1. Daniel Rodrigues Blanco
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  2. Marcelo Ricardo Vicari
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  3. Roberto Laridondo Lui
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  4. Luiz Antônio Carlos Bertollo
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  5. Josiane Baccarin Traldi
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  6. Orlando Moreira-Filho
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Correspondence to Daniel Rodrigues Blanco.

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Blanco, D.R., Vicari, M.R., Lui, R.L. et al. The role of the Robertsonian rearrangements in the origin of the XX/XY1Y2 sex chromosome system and in the chromosomal differentiation in Harttia species (Siluriformes, Loricariidae). Rev Fish Biol Fisheries 23, 127–134 (2013). https://doi.org/10.1007/s11160-012-9283-5

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  • DOI: https://doi.org/10.1007/s11160-012-9283-5

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