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Chromosomal studies in Crenicichla lepidota and Australoheros facetus (Cichlidae, Perciformes) from extreme Southern Brazil

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Abstract

Fishes of the family Cichlidae generally show low karyotype variability. Nevertheless, karyotype variants have been identified within some genera, providing information about their evolutionary history. In the present study, karyotype characteristics of Crenicichla lepidota and Australoheros facetus, two sympatric species found in the São Gonçalo-Mangueira basin, were studied. Besides conventional procedures, double fluorochromes staining chromomycin A3/DAPI and fluorescent in situ hybridization (FISH) with rDNA probes were also used. Both species presented 2n = 48 chromosomes, but karyotypes were differentiated by fundamental number, which was equal to 70 in A. facetus and 56 in C. lepidota. Similar heterochromatin distribution patterns were also observed on the pericentromeric region of most chromosomes, although C. lepidota presented an additional heterochromatic block in the first pair. The Ag-NORs, 18S rDNA probe and CMA3/DAPI were coincident in location on the first and second pairs of C. lepidota and A. facetus, respectively. The minor rDNA loci (5S rDNA) were found in four sites located on two distinct chromosomal pairs in C. lepidota. Although the data obtained here to C. lepidota and to A. facetus show chromosomal characteristics considered ancestral to the family, new data are presented to both species. Additionally, this study corroborates the hypothesis in which evolutionary processes like non-Robertsonian rearrangements are involved in the diversification of the major groups of Neotropical Cichlidae. Thus, the karyotype diversification observed in A. facetus have the high fundamental number pathway while C. lepidota has others evolutionary chromosomal mechanisms.

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Acknowledgments

The authors are grateful to Instituto Brasileiro do Meio Ambiente and Recursos Naturais Renováveis (MMA/IBAMA) for authorizing the capture of the fishes. This study was financed by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and the Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná). We also thank the team of Professor Luiz Malabarba, from Universidade Federal do Rio Grande do Sul, for identifying and cataloguing specimens.

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Authors and Affiliations

  1. Departamento de Genética, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, Caixa Postal 19071, Curitiba, Paraná, 81531-990, Brazil

    Giselle Perazzo & Marta Margarete Cestari

  2. Departamento de Biologia, Faculdade Estadual de Filosofia, Ciências e Letras de União da Vitória, Praça Coronel Amazonas, Centro, União da Vitória, Paraná, 84600-000, Brazil

    Rafael Bueno Noleto

  3. Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Avenida Carlos Cavalcanti, Caixa Postal 4748, Uvaranas, Ponta Grossa, Paraná, 84030-900, Brazil

    Marcelo Ricardo Vicari

  4. Instituto de Biociências, Universidade Federal do Rio Grande, Avenida Itália, Km 8, Campus Carreiros, Caixa Postal 474, Rio Grande, Rio Grande do Sul, 96201-900, Brazil

    Patricia Coelho Machado & Adriana Gava

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  1. Giselle Perazzo
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  2. Rafael Bueno Noleto
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  3. Marcelo Ricardo Vicari
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Correspondence to Giselle Perazzo.

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Perazzo, G., Noleto, R.B., Vicari, M.R. et al. Chromosomal studies in Crenicichla lepidota and Australoheros facetus (Cichlidae, Perciformes) from extreme Southern Brazil. Rev Fish Biol Fisheries 21, 509–515 (2011). https://doi.org/10.1007/s11160-010-9170-x

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  • DOI: https://doi.org/10.1007/s11160-010-9170-x

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