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Suppression of Cell Wall Degrading Enzymes and their Encoding Genes in Button Mushrooms (Agaricus bisporus) by CaCl2 and Citric Acid

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Abstract

Fresh button mushrooms (Agaricus bisporus) were harvested and treated with a solution of 1.5% CaCl2 + 0.5% citric acid and stored for 16 days at 12 °C. The effects of this treatment on firmness, weight, color, cell wall compositions (cellulose and chitin) and cell wall degrading enzymes (cel1ulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase) were investigated during post-harvest storage. The expressions of major genes (Cel1, Glu1, Chi1 and PAL1) involved in cell wall degradation during post-harvest storage were also monitored. The results revealed that the post-harvest chemical treatment maintained better firmness, weight, color and inhibited cellulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase activities. These findings showed that the down-regulation of cell wall degrading enzymes is a possible mechanism that delays the softening of button mushrooms by the application of combined chemical treatment.

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Abbreviations

PAL:

Phenylalaine ammonialyse

CK:

Control sample

T:

Treated sample

MAP:

Modified atmospheric packing

w/v :

Weight/Volume

qPCR:

Quantitative polymerase chain reaction

cDNA:

Complimentary deoxyribonucleic acid

RNA:

Ribonucleic acid

References

  1. Beelman RB, Royse DJ, Chikthimmah N (2003) Bioactive components in button mushroom (Agaricus bisporus) (J. Lge) Imbach (Agaricomycetideae) of nutritional, medicinal, and biological importance (Review). Int J Med Mush 5(4):321–327

  2. Kamada T, Hamada Y, Takemaru T (1982) Autolysis in vitro of the stipe cell wall in Coprinus rnacrorhizus. J Gen Microbiol 128:1041–1046

    CAS  Google Scholar 

  3. Ares G, Lareo C, Lema P (2007) Modified atmosphere packaging for postharvest storage of mushrooms. A review. Fresh Produce 1(1):32–40

    Google Scholar 

  4. Zivanovic S, Busher R, Kim K (2000) Textural changes in mushrooms (Agaricus bisporus) associated with tissue ultrastructure and composition. J Food Sci 65:1404–1408

    Article  CAS  Google Scholar 

  5. Jiang T, Wang Q, Xu S, Jahangir MM, Ying T (2010) Structure and composition changes in the cell wall in relation to texture of shiitake mushrooms (Lentinula edodes) stored in modified atmosphere packaging. J Sci Food Agric 90:742–749

    CAS  Google Scholar 

  6. Zivanovic S, Buescher R, Kim S (2003) Mushroom texture, cell wall composition, color, and ultrastructure as affected by pH and temperature. J Food Sci 68:1860–1865

    Article  CAS  Google Scholar 

  7. Wessels J, Mol P, Sietsma J, Vermeulen C (1990) Wall structure, wall growth, and fungal cell morphogenesis, biochemistry of cell walls and membranes in fungi. Springer, pp 81–95.

  8. Sakamoto Y, Nakade K, Konno N, Sato T (2012) Senescence of the Lentinula edodes fruiting body after harvesting. Food quality. InTech North America, New York

    Google Scholar 

  9. Rosales R, Fernandez-Caballero C, Romero I, Escribano MI, Merodio C, Sanchez-Ballesta MT (2013) Molecular analysis of the improvement in rachis quality by high CO2 levels in table grapes stored at low temperature. Postharvest Biol Technol 77:50–58

    Article  CAS  Google Scholar 

  10. Brennan MH, Gormley RT (1998) Extending the shelf life of fresh sliced mushrooms. The National Food Centre Research Report No. 2. Martine H. Brennan and T. Ronan Gormley. Dublin; Teagasc, ISBN 1901138402

  11. Paul U, Juan V, Jette J (2016) Impact on vitamin D2, vitamin D4 and Agaritine in Agaricus bisporus mushrooms after artificial and natural solar UV light exposure. Plant Foods Hum Nutr 71:314–321

    Article  Google Scholar 

  12. Kang MK, Jeong HC, Mosbah MK, Elizabeth HJ, John AJ (2013) Pre-harvest methyl jasmonate treatment enhances cauliflower chemoprotective attributes without a loss in postharvest quality. Plant Foods Hum Nutr 68:113–117

    Article  Google Scholar 

  13. Jafri M, Jha A, Bunkar DS, Ram RC (2013) Quality retention of oyster mushrooms (Pleurotus florida) by a combination of chemical treatments and modified atmosphere packaging. Postharvest Biol Technol 76:112–118

    Article  CAS  Google Scholar 

  14. Khan ZU, Bu J, Khan NM, Khan RU, Jiang Z, Mou W, Lou Z, Mao L, Ying T (2014) Integrated treatment of CaCl2, citric acid and sorbitol reduces loss of quality of button mushroom (Agaricus bisporus) during postharvest storage. J Food Process Preserv 39:2008–2016. doi:10.1111/jfpp.12441

  15. Figueroa CR, Opazo MC, Vera P, Arriagada O, Diaz M, Moya-Leon MA (2012) Effect of postharvest treatment of calcium and auxin on cell wall composition and expression of cell wall-modifying genes in the Chilean strawberry (Fragaria chiloensis) fruit. Food Chem 32:2014–2022

    Article  Google Scholar 

  16. Khan ZU, Aisikaer G, Khan RU, Bu J, Jiang Z, Ni Z, Ying T (2014) Effects of composite chemical pretreatment on maintaining quality in button mushrooms (Agaricus bisporus) during postharvest storage. Postharvest Biol Technol 95:36–41

    Article  CAS  Google Scholar 

  17. Dm U (1969) Semimicro determination of cellulose in biological materials. Anal Biochem 32(3):420–424

    Article  Google Scholar 

  18. Durbin ML, Lewis LN (1988) Cellulases in Phaseolus vulgaris. Methods Enzymol 160:342–351

    Article  CAS  Google Scholar 

  19. Pan SQ, Ye XS, Kuc J (1991) Association of beta-1,3-glucanase activity and isoform pattern with systemic resistance to blue mold in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus. Physiol Mol Plant Pathol 39:25–39

    Article  CAS  Google Scholar 

  20. Boller T, Mauch F (1988) Colorimetric assay for chitinase. Methods Enzymol 161:430–435

    Article  CAS  Google Scholar 

  21. Koukol J, Conn EE (1961) The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem 236:2692–2698

    CAS  Google Scholar 

  22. Briones GL, Varoquaux P, Chambroy Y, Bouquant J, Bureau G, Pascat B (1992) Storage of common mushroom under controlled atmospheres. Int J Food Sci Technol 27:493–505

    Article  Google Scholar 

  23. Koushki M, Abras SK, Mohammadi M, Hadian Z, Poorfallah NB, Sharayei P, Mortazavian AM (2011) Physicochemical properties of mushrooms as affected by modified atmosphere packaging and CaCl2 dipping. Afr J Agric Res 6(24):5414–5421

  24. Waghmare RB, Annapure US (2013) Combined effect of chemical treatment and/or modified atmosphere packaging (MAP) on quality of fresh-cut papaya. Postharvest Biol Technol 85:147–153

    Article  CAS  Google Scholar 

  25. Brummell DA, Harpster MH (2001) Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol 47:311–340

    Article  CAS  Google Scholar 

  26. Eissa HAA (2007) Effect of chitosan coating on shelf life and quality of fresh-cut mushroom. J Food Qual 30:623–645

    Article  CAS  Google Scholar 

  27. Manganaris GA, Vicente AR, Crisosto CH, Labavitch JM (2007) Effect of dips in a 1-methylcyclopropene-generating solution on 'Harrow Sun' plums stored under different temperature regimes. J Sci Food Agric 55:7015–7020

    Article  CAS  Google Scholar 

  28. Artes F, Castaner M, Gil MI (1998) Review: enzymatic browning in minimally processed fruit and vegetables. Food Sci Technol Int 4:377–389

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful for the financial supports of National Natural Science Foundation of China (Project Number: 31171766) and the Foundation for the Program of Key Innovative Research Team of Zhejiang Province (2009R50036).

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Authors and Affiliations

  1. College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Zia Ullah Khan, Li Jiayin, Wangshu Mou, Dongdong Li, Yansheng Wang, Simin Feng, Zisheng Luo, Linchun Mao & Tiejin Ying

  2. Department of Agriculture, Abdul Wali Khan University, Mardan, Khyber-Pakhtunkhwa, 23200, Pakistan

    Zia Ullah Khan

  3. Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), Khyber-Pakhtunkhwa, 18000, Pakistan

    Nasir Mehmood Khan

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  1. Zia Ullah Khan
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  2. Li Jiayin
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  3. Nasir Mehmood Khan
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Correspondence to Zia Ullah Khan.

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Khan, Z.U., Jiayin, L., Khan, N.M. et al. Suppression of Cell Wall Degrading Enzymes and their Encoding Genes in Button Mushrooms (Agaricus bisporus) by CaCl2 and Citric Acid. Plant Foods Hum Nutr 72, 54–59 (2017). https://doi.org/10.1007/s11130-016-0588-8

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  • DOI: https://doi.org/10.1007/s11130-016-0588-8

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