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Evaluation of the Antioxidant and Antiproliferative Effects of Three Peptide Fractions of Germinated Soybeans on Breast and Cervical Cancer Cell Lines

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Abstract

Soybeans are an important source of bioactive molecules, such as peptides, which generation can improve through germination. In this study, the antioxidant and antiproliferative activities of three peptide fractions (>10 kDa, 5–10 kDa and <5 kDa) that were obtained by ultrafiltration of soybean protein hydrolysate after six days of germination were evaluated. The antioxidant activities of the peptide fractions were assessed by reducing power, Cu+2 and Fe+2 chelation and OH· scavenging assays, whereas their antiproliferative effects against cervical (HeLa, SiHa, CasKi) and breast (MCF7 and MDA-MB-231) cancer cell lines were evaluated by the MTT assay. Apoptosis was determined by Hoechst-PI staining. The most active peptide fraction (MAPF) was the >10 kDa fraction, which showed the greatest antioxidant and antiproliferative activity. The most sensitive cancer cell lines were the HeLa, CasKi and MDA-MB-231 cells, which had IC50 values of 16.2, 14.3 and 15.2 mg/mL, respectively, and apoptotic indices above 50 % after 6 or 8 h of exposure. The effect of MAPF on normal cells (HaCaT) was minimal. The amino acid composition of MAPF was characterized by high proline, phenylalanine and tyrosine content, and MALDI-TOF/TOF analysis showed six signals with molecular weights of 12 to 42 kDa.

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Acknowledgments

This research was financed by the Project SEP-CONACyT 242860, and by grants from the Instituto Politécnico Nacional (IPN) and the COFAA-IPN.

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Authors and Affiliations

  1. Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Unidad Profesional “Adolfo López Mateos”, Calle Wilfrido Massieu esquina Cda. Manuel Stampa. C.P, 07738, Ciudad de México, México

    González-Montoya Marcela, Robles-Ramírez María del Carmen & Mora-Escobedo Rosalva

  2. Lab. de Citopatologia Ambiental, Depto. de Morfología. Escuela Nacional de Ciencias Biológicas, Ciudad de México, México

    Ramón-Gallegos Eva

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  1. González-Montoya Marcela
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  2. Ramón-Gallegos Eva
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  3. Robles-Ramírez María del Carmen
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  4. Mora-Escobedo Rosalva
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Correspondence to Mora-Escobedo Rosalva.

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Marcela, GM., Eva, RG., del Carmen, RR.M. et al. Evaluation of the Antioxidant and Antiproliferative Effects of Three Peptide Fractions of Germinated Soybeans on Breast and Cervical Cancer Cell Lines. Plant Foods Hum Nutr 71, 368–374 (2016). https://doi.org/10.1007/s11130-016-0568-z

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  • DOI: https://doi.org/10.1007/s11130-016-0568-z

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