Abstract
The effect of the postharvest treatment of methyl jasmonate enantiomers in conjunction with ethanol on bioformation of resveratrol and quercetin glycosides in grapes was evaluated. The antioxidant activity of treated grape extracts as compared with untreated extracts was also assayed. Exogenous (−)-methyl jasmonate in combination with ethanol induced a significant increase in the levels of resveratrol (from 27 to 39 μg g−1), quercetin-3-O-glucoside (from 59 to 136 μg g−1), quercetin-3-O-galactoside (from 398 to 807 μg g−1) and quercetin-3-O-rutinoside (from 23 to 43 μg g−1). (+)-Methyl jasmonate with ethanol also resulted in increase of quercetin-3-O-glucoside and quercetin-3-O-rutinoside. However, no (+)-methyl jasmonate effect was observed for resveratrol and quercetin-3-O-galactoside. Both (−)- and (+)-methyl jasmonate treatments provided with extracts with higher antioxidant activity. From the results found in the present work postharvest treatment with (−)-methyl jasmonate in conjunction with ethanol is proposed as a mean to obtain polyphenol-enriched grape extracts with improved antioxidant properties. The procedure here developed is proposed as a mean to obtain functional grapes. Extracts obtained from grapes treated with (−)-methyl jasmonate with ethanol can be particularly useful for industry due to their high antioxidant capacity.
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Chanjirakul K, Wang SY, Wang CY, Siriphanich J (2006) Effect of natural volatile compounds on antioxidant capacity and antioxidant enzymes in rapsberries. Postharvest Biol Technol 40:106–115
Ayala-Zavala JF, Wang SY, Wang CY, González-Aguilar GA (2005) Methyl jasmonate in conjunction with ethanol treatment increases antioxidant capacity, volatile compounds and postharvest life of strawberry fruit. Eur Food Res Technol 221:731–738
Mo Ku K, Choi J-H, Kushad MM, Jeffery EH, Juvik JA (2013) Pre-harvest methyl jasmonate treatment enhances cauliflower chemoprotective attributes without a loss in postharvest quality. Plant Foods Hum Nutr 68:113–117
Ruiz-Garcia Y, Romero-Cascales I, Gil-Muñoz R, Fernández-Fernández JI, Lopez-Roca JM, Gómez-Plaza E (2012) Improving grape phenolic content and wine chromatic characteristics through the use of two different elicitors: methyl jasmonate versus benzothiadiazole. J Agric Food Chem 60:1283–1290
Wang SY, Bowman L, Ding M (2008) Methyl jasmonate enhances antioxidant activity and flavonoid content in blackberries (Rubus sp.) and promotes antiproliferation of human cancer cells. Food Chem 107:1261–1269
Verzulli S, Civardi S, Ferrari F, Bavaresco L (2007) Methyl jasmonate treatment as a trigger of resveratrol synthesis in cultivated grapevine. Am J Enol Vitic 58:530–533
Acree TE, Nishida R, Fukami H (1985) Odor thresholds of the stereoisomers of methyl jasmonate. J Agric Food Chem 33:425–427
Koda Y, Kikuta Y, Kitahara Nishi T, Mori K (1992) Comparisons of various biological activities of methyl jasmonate. Phytochemistry 31:1111–1114
de la Peña MF, Blanch GP, Ruiz del Castillo ML (2010a) (+)-methyl jasmonate-induced bioformation of myricetin, quercetin and kaempferol in red raspberries. J Agric Food Chem 58:11639–11644
Flores G, Blanch GP, Ruiz del Castillo ML (2013) Postharvest treatment of red raspberry fruits with pure methyl jasmonate stereoisomers isolated by chiral HPLC. Food Chem 141:2982–2987
de la Peña MF, Blanch GP, Ruiz del Castillo ML (2010b) Effect of (−)- and (+)-methyl jasmonate on the bioformation of aroma-active esters in strawberry fruits. Eur Food Res Technol 231:829–834
Darne G (1993) New hypothesis on anthocyanin biosynthesis in berries and leaves in grapevine. Vitis 32:77–85
Manach C, Scalbert A, Morand C, Remesy C, Jiménez L (2004) Polyphenols: food sources and bioavailability studies. Am J Clin Nutr 81:230S–242S
Manach C, Williamnson G, Morand C, Scalbert A, Remesey C (2005) Bioavailability and bioefficacy of polyphenols in humans I. Review of 97 bioavailability studies. Am J Clin Nutr 81:230S–242S
Orsolic N, Knezevic AH, Sver L, Terzic S, Basic I (2004) Immuno-modulatory and antimetastatic action of propolis and related polyphenolic compounds. J Ethnopharmacol 94:307–315
Elattar TMA, Virji AS (2000) The inhibitory effects of curcumin, genistein, quercetin and cisplatin on the growth of oral cancer cells in vitro. Anticancer Res 20:1733–1738
Perez-Vizcaino F, Bishop-Bailley D, Lodi F, Duarte J, Cogolludo A, Moreno L, Bosca L, Mitchell JA, Warner TD (2006) The flavonoid quercetin induces apoptosis and inhibits JNK activation in intimal vascular smooth muscle cells. Biochem Biophys Res Commun 346:919–925
Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM (1997) Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275:218–220
Bradamante S, Barenghi L, Villa A (2004) Cardiovascular protective effects of resveratrol. Cardiov Drug Rev 22:169–188
Sinha K, Chaudhary G, Gupta YK (2002) Protective effects of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats. Life Sci 71:655–665
Flores G, Blanch GP, Ruiz del Castillo ML (2015) Postharvest treatment with (−) and (+)-methyl jasmonate stimulates anthocyanin accumulation in grapes. LWT Food Sci Technol 62:807–812
Smith RC, Reeves JC, Dage RC, Schnettler RA (1987) Antioxidant properties of 2-imidazolones and 2-imidazolthiones. Biochem Pharmacol 36:1457–1460
Kim HJ, Chen F, Wang X, Choi JH (2006) Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.). J Agric Food Chem 54:7263–7269
Heredia JB, Cisneros-Zevallos L (2009) The effect of exogenous ethylene and methyl jasmonate on pal activity, phenolic profiles and antioxidant capacity, phenolic profiles and antioxidant capactity of carrots (Daucus carota) under different wounding intensities. Postharvest Biol Technol 51:242–249
Cao S, Zheng Y, Wang K, Jin P, Rui H (2009) Methyl jasmonate reduces chilling injury and enhances antioxidant enzyme activity in postharvest loquat fruit. Food Chem 115:1458–1463
Flores G, Ruiz del Castillo ML (2014) Influence of preharvest and postharvest methyl jasmonate treatments on flavonoid content and metabolomic enzymes in red raspberry. Postharvest Biol Technol 97:77–82
Acknowledgments
Authors thank the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER program (research project S2013/ABI-3028, AVANSECAL-CM) for financial support. Dra. Gema Flores thanks CSIC for her JAE-Doc contract.
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Flores, G., del Castillo, M.L.R. New Procedure to Obtain Polyphenol-Enriched Grapes Based on the Use of Chemical Elicitors. Plant Foods Hum Nutr 71, 239–244 (2016). https://doi.org/10.1007/s11130-016-0546-5
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DOI: https://doi.org/10.1007/s11130-016-0546-5