Plant Foods for Human Nutrition

, Volume 67, Issue 2, pp 156–161

Phenolic Compounds from Maté (Ilex paraguariensis) Inhibit Adipogenesis in 3T3-L1 Preadipocytes

  • Grace Gosmann
  • Adriana Gregory Barlette
  • Tabitha Dhamer
  • Demétrius P. Arçari
  • Juliana Carvalho Santos
  • Eloá Ramalho de Camargo
  • Simone Acedo
  • Alessandra Gambero
  • Simone Cristina Baggio Gnoatto
  • Marcelo Lima Ribeiro
Original Paper

DOI: 10.1007/s11130-012-0289-x

Cite this article as:
Gosmann, G., Barlette, A.G., Dhamer, T. et al. Plant Foods Hum Nutr (2012) 67: 156. doi:10.1007/s11130-012-0289-x

Abstract

Leaves of Ilex paraguariensis are used to prepare a tea known as maté which is a common beverage in several South American countries. The ethanol extract was fractionated to identify the compounds responsible for the anti-adipogenic activity in 3T3-L1 cells. Extracts of both fresh and dried maté leaves were subjected to column chromatography using molecular permeation to obtain the saponin (20 % yields) and the polyphenol extracts (40 % yields) from the fresh and dried leaves. The phenolic content was determined using high-performance liquid chromatography analysis and the Folin-Ciocalteau method. Also, maté extracts (50 μg/ml to 1,000 μg/ml) did not display citotoxicity using MTT. The polyphenol extract from the dried leaves was the most effective (50 μg/ml) in the inhibition of triglyceride accumulation in 3T3-L1 adipocytes, and rutin (100 μg/ml) likely accounted for a large portion of this activity. Additionally, maté extracts had a modulatory effect on the expression of genes related to the adipogenesis as PPARγ2, leptin, TNF-α and C/EBPα.

Keywords

Ilex paraguariensisMatéAdipogenesisPolyphenolsSaponinMTT

Abbreviations

DMEM

Dulbecco’s modified Eagle’s medium

IBMX

3-isobutyl-1-methylxanthine

TNFα

Tumor necrosis factor-alpha

FE

Fresh leaf ethanol extract

DE

Dried leaf ethanol extract

Supplementary material

11130_2012_289_MOESM1_ESM.pdf (47 kb)
ESM 1(PDF 47 kb)

Copyright information

© Springer Science+Business Media, Inc. 2012

Authors and Affiliations

  • Grace Gosmann
    • 1
  • Adriana Gregory Barlette
    • 1
    • 2
  • Tabitha Dhamer
    • 1
  • Demétrius P. Arçari
    • 2
  • Juliana Carvalho Santos
    • 2
  • Eloá Ramalho de Camargo
    • 2
  • Simone Acedo
    • 2
  • Alessandra Gambero
    • 2
  • Simone Cristina Baggio Gnoatto
    • 1
  • Marcelo Lima Ribeiro
    • 2
  1. 1.Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Unidade Integrada de Farmacologia e GastroenterologiaUniversidade São FranciscoBragança PaulistaBrazil