Photosynthesis Research

, Volume 131, Issue 1, pp 65–77

A two-component nonphotochemical fluorescence quenching in eustigmatophyte algae

Original Article

DOI: 10.1007/s11120-016-0299-x

Cite this article as:
Bína, D., Bouda, K. & Litvín, R. Photosynth Res (2017) 131: 65. doi:10.1007/s11120-016-0299-x

Abstract

Eustigmatophyte algae represent an interesting model system for the study of the regulation of the excitation energy flow due to their use of violaxanthin both as a major light-harvesting pigment and as the basis of xanthophyll cycle. Fluorescence induction kinetics was studied in an oleaginous marine alga Nannochloropsis oceanica. Nonphotochemical fluorescence quenching was analyzed in detail with respect to the state of the cellular xanthophyll pool. Two components of nonphotochemical fluorescence quenching (NPQ), both dependent on the presence of zeaxanthin, were clearly resolved, denoted as slow and fast NPQ based on kinetics of their formation. The slow component was shown to be in direct proportion to the amount of zeaxanthin, while the fast NPQ component was transiently induced in the presence of membrane potential on subsecond timescales. The applicability of these observations to other eustigmatophyte species is demonstrated by measurements of other representatives of this algal group, both marine and freshwater.

Keywords

Chl a fluorescence Nonphotochemical quenching Xanthophyll cycle Nannochloropsis Eustigmatophyceae 

Abbreviations

AL

Actinic illumination

Ant

Antheraxanthin

Chl

Chlorophyll

DCMU

3-(3,4-Dichlorophenyl)-1,1-dimethylurea

DEPS

Xanthophyll pool de-epoxidation state

NPQ

Nonphotochemical (Chl a fluorescence) quenching

PSII

Photosystem II

SP

Saturating pulse

Vio

Violaxanthin

Zea

Zeaxanthin

Supplementary material

11120_2016_299_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1125 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Institute of Plant Molecular BiologyBiology Centre CASČeské BudějoviceCzech Republic
  2. 2.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic

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