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Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics

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Abstract

Using radioactively labelled amino acids to investigate repair of photoinactivated photosystem II (PS II) gives only a relative rate of repair, while using chlorophyll fluorescence parameters yields a repair rate coefficient for an undefined, variable location within the leaf tissue. Here, we report on a whole-tissue determination of the rate coefficient of photoinactivation k i , and that of repair k r in cotton leaf discs. The method assays functional PS II via a P700 kinetics area associated with PS I, as induced by a single-turnover, saturating flash superimposed on continuous background far-red light. The P700 kinetics area, directly proportional to the oxygen yield per single-turnover, saturating flash, was used to obtain both k i and k r . The value of k i , directly proportional to irradiance, was slightly higher when CO2 diffusion into the abaxial surface (richer in stomata) was blocked by contact with water. The value of k r , sizable in darkness, changed in the light depending on which surface was blocked by contact with water. When the abaxial surface was blocked, k r first peaked at moderate irradiance and then decreased at high irradiance. When the adaxial surface was blocked, k r first increased at low irradiance, then plateaued, before increasing markedly at high irradiance. At the highest irradiance, k r differed by an order of magnitude between the two orientations, attributable to different extents of oxidative stress affecting repair (Nishiyama et al., EMBO J 20: 5587–5594, 2001). The method is a whole-tissue, convenient determination of the rate coefficient of photoinactivation k i and that of repair k r .

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Abbreviations

Chl:

Chlorophyll

f :

Functional fraction of PS II

F o , F m :

Minimum and maximum Chl fluorescence yield of a dark-adapted leaf, respectively

F v  = (F m –F o ):

Variable fluorescence

k i , k r :

Rate coefficient of photoinactivation and repair, respectively

P700:

Special Chl pair in the PSI reaction centre

PI:

Photoinactivation

PS:

Photosystem

ROS:

Reactive oxygen species

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Acknowledgments

The support of this work by an Australian Research Council Grant (DP1093827) awarded to W. S. C., the Joint Funds of the National Natural Science Foundation of China Grant (No. U1203283) and a National Key Technology R and D Program of China Grant (2007BAD44B07) to Z. W. F., and a Knowledge Innovation Program of the Chinese Academy of Sciences grant (KZCX2-XB3-09-02) to D. -Y. F. is gratefully acknowledged. W. S. C. values the friendship with Gov that started at a meeting in China in 1984. W. S. C. has long admired the work of Pierre J., as well as the life and work of Pierre’s grandparents.

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Authors and Affiliations

  1. The Key Laboratory of Oasis Eco-agriculture, Xinjiang Production and Construction Group, Shihezi University, Shihezi, 832003, People’s Republic of China

    Yuan-Yuan Hu & Wang-Feng Zhang

  2. Division of Plant Science, Research School of Biology, The Australian National University, R.N. Robertson Building (46), Canberra, ACT, 0200, Australia

    Yuan-Yuan Hu, Da-Yong Fan & Wah Soon Chow

  3. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China

    Da-Yong Fan

  4. Consiglio per la Ricerca e la sperimentazione in Agricoltura [Research Unit for Agriculture in Dry Environments], 70125, Bari, Italy

    Pasquale Losciale

  5. Dipartimento di Scienze Agrarie, University of Bologna, 40127, Bologna, Italy

    Pasquale Losciale

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  1. Yuan-Yuan Hu
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  2. Da-Yong Fan
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  3. Pasquale Losciale
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  4. Wah Soon Chow
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  5. Wang-Feng Zhang
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Correspondence to Wah Soon Chow or Wang-Feng Zhang.

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Yuan-Yuan Hu and Da-Yong Fan contributed equally to this study.

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Hu, YY., Fan, DY., Losciale, P. et al. Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics. Photosynth Res 117, 517–528 (2013). https://doi.org/10.1007/s11120-013-9822-5

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