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Identification and Expression Analysis of the 14-3-3 Gene Family in the Mulberry Tree

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Abstract

The 14-3-3 protein family regulates diverse physiological processes in plants. In this study, we identified eight 14-3-3 genes (designated MaGF14a to MaGF14h) from the mulberry tree. The phylogenetic tree demonstrated that MaGF14s can be divided into two major groups. Tissue-specific expression profiles indicated that the eight MaGF14s were expressed in all organs and they were most highly expressed in the female flower. We also detected different expression of the eight MaGF14s during seed germination and flower development. MaGF14a, MaGF14c, and MaGF14h were strongly expressed in the first 3 days after seed germination, while the MaGF14b, MaGF14d, MaGF14e, MaGF14f, and MaGF14g isoforms were expressed highly in the later period. All the MaGF14s were expressed low during development of male flowers and fruits. These findings indicate that MaGF14s play important roles in the growth and development of the mulberry tree.

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Abbreviations

Ma:

Morus alba

MaGF14s:

14-3-3 gene family of mulberry

BLAST:

Basic Local Alignment Search Tool

CDS:

Coding sequence

qRT-PCR:

Real-time quantitative polymerase chain reaction

NCBI:

National Center for Biotechnology Information

EMBL:

European Molecular Biology Laboratory

DNase I:

Deoxyribonuclease I

TM:

Melting temperature

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Acknowledgments

This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110182120024), the National Natural Science Foundation of China (Grant No. 31101769), and the Fundamental Research Funds for the Central Universities (No. XDJK2 014C065).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Biotechnology, Southwest University, Chongqing, 400716, China

    Yan Yang, Maode Yu, Fengxiang Xu, Yasheng Yu, Changying Liu, Jun Li & Xiling Wang

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  1. Yan Yang
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  2. Maode Yu
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  3. Fengxiang Xu
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  4. Yasheng Yu
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  7. Xiling Wang
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Correspondence to Xiling Wang.

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Yang, Y., Yu, M., Xu, F. et al. Identification and Expression Analysis of the 14-3-3 Gene Family in the Mulberry Tree. Plant Mol Biol Rep 33, 1815–1824 (2015). https://doi.org/10.1007/s11105-015-0877-7

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