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Isolation and Identification of Differentially Expressed Genes from Wheat in Response to Blumeria graminis f. sp. tritici (Bgt)

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Abstract

Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a serious wheat disease both in China and worldwide. Finding and cloning the key resistance genes to Bgt are important for wheat breeding. In this study, 23 differentially expressed genes were isolated by GeneFishing from the hybrid wheat alien disomic addition line germplasm SN6306, which is highly resistant to powdery mildew. SN6306 originated from the intergeneric hybridization between wheat Yannong 15 and Elytrigia intermedium (Host) Nevski. Among the 23 genes, a gene encoding for auxin-repressed protein (TaARP1) was selected for further study, and its full-length complementary DNA (cDNA) and DNA sequences were obtained. The function of TaARP1 was investigated by real-time quantitative reverse transcription PCR and Barley Stripe Mosaic Virus-mediated gene silencing. Results showed that, after inoculation with Bgt, the expression of TaARP1 was upregulated more than ten times compared with non-inoculated controls. Ten days after inoculation with powdery mildew, BSMV:TaARP1 leaves clearly showed higher susceptibility to mildew infections than BSMV:00 leaves. Powdery mildew colonies and mycelia development were analyzed by microscopic observation. These data suggested that TaARP1 may be involved in the response to Bgt and may be a potential resistance gene to wheat powdery mildew.

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Abbreviations

ACP:

Annealing control primer

ARP:

Auxin-repressed protein

BSMV:

Barley Stripe Mosaic Virus

CTAB:

Cetyltrimethylammonium bromide

DEGs:

Differentially expressed genes

PCR:

Polymerase chain reaction

VIGS:

Virus-induced gene silencing

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Acknowledgments

The authors are grateful for research grants from the National Natural Science Foundation of China (31171552) and thank Prof. Dawei Li (College of Biological Sciences, China Agricultural University) for sending us the BSMV-VIGS system.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an, 271018, China

    Jing Song, Zubiao Niu, Quanquan Li, Yinguang Bao, Xin Ma, Honggang Wang, Lingrang Kong & Deshun Feng

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  1. Jing Song
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  2. Zubiao Niu
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  3. Quanquan Li
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  4. Yinguang Bao
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  5. Xin Ma
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  6. Honggang Wang
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  7. Lingrang Kong
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  8. Deshun Feng
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Corresponding author

Correspondence to Deshun Feng.

Additional information

Jing Song, Zubiao Niu, and Quanquan Li contributed equally to this work.

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Song, J., Niu, Z., Li, Q. et al. Isolation and Identification of Differentially Expressed Genes from Wheat in Response to Blumeria graminis f. sp. tritici (Bgt). Plant Mol Biol Rep 33, 1371–1380 (2015). https://doi.org/10.1007/s11105-014-0838-6

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  • DOI: https://doi.org/10.1007/s11105-014-0838-6

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