Plant Molecular Biology Reporter

, Volume 33, Issue 2, pp 221–238

Pea p68 Imparts Salinity Stress Tolerance in Rice by Scavenging of ROS-Mediated H2O2 and Interacts with Argonaute

  • Mst. Sufara Akhter Banu
  • Kazi Md. Kamrul Huda
  • Ranjan Kumar Sahoo
  • Bharti Garg
  • Suresh Tula
  • S. M. Shahinul Islam
  • Renu Tuteja
  • Narendra Tuteja
Original Paper

DOI: 10.1007/s11105-014-0748-7

Cite this article as:
Banu, M.S.A., Huda, K.M.K., Sahoo, R.K. et al. Plant Mol Biol Rep (2015) 33: 221. doi:10.1007/s11105-014-0748-7

Abstract

The p68, a prototypic member of DEAD-box protein family, is involved in pre-mRNA splicing, RNA-induced silencing and transcription regulation. However, the role of plant p68 in stress tolerance and molecular targets responsible for this has not been reported. Here, we report the isolation and characterization of salinity-induced pea p68 (Psp68). The expression of Psp68 was more in aerial parts as compared to the roots in response to the abiotic stress. The transgenic-overexpressing Psp68 showed enhanced tolerance to salinity stress by efficiently attenuating ionic adjustment and scavenging reactive oxygen species (ROS). The enhanced tolerance was further confirmed by observing several physiological indices. Psp68-overexpressing rice accumulated higher K+ and Ca2+ and lower Na+ level as compared to the wild-type (WT) plants. Furthermore, Psp68 interacts with pea argonaute (AGO1), a catalytic component of the RNA-induced silencing complex (RISC) responsible for the gene silencing. The microarray analysis showed that Psp68 regulates many transcripts involved in the abiotic and oxidative stress responses as well as gene-silencing mechanisms in rice. Thus, the Psp68 functions as a molecular switch in different signaling pathways leading to stress tolerance. Overall, Psp68 may serve as a useful biotechnological tool for the improvement of stress tolerance crops.

Keywords

Abiotic stress Antioxidant Argonaute Microarray analysis Oryza sativa Pea p68 ROS Salinity stress 

Supplementary material

11105_2014_748_MOESM1_ESM.pdf (556 kb)
Fig. S1(PDF 555 kb)
11105_2014_748_MOESM2_ESM.pdf (28 kb)
Fig. S2(PDF 27 kb)
11105_2014_748_MOESM3_ESM.pdf (111 kb)
Fig. S3(PDF 110 kb)
11105_2014_748_MOESM4_ESM.pdf (27 kb)
Table S1(PDF 26 kb)
11105_2014_748_MOESM5_ESM.pdf (19 kb)
Table S2(PDF 19 kb)

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mst. Sufara Akhter Banu
    • 1
  • Kazi Md. Kamrul Huda
    • 1
  • Ranjan Kumar Sahoo
    • 1
  • Bharti Garg
    • 1
  • Suresh Tula
    • 1
  • S. M. Shahinul Islam
    • 1
  • Renu Tuteja
    • 1
  • Narendra Tuteja
    • 1
  1. 1.International Centre for Genetic Engineering and Biotechnology (ICGEB)New DelhiIndia