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Characterization of a Novel NBS-LRR Gene Involved in Bacterial Blight Resistance in Rice

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Abstract

Nucleotide-binding site leucine-rich repeat (NB-LRR) genes play important roles in plant disease resistance. Due to its similarity to Rp1 in maize (Zea mays), a novel NB-LRR gene was isolated from rice and designated as OsRP1L1 (Oryza sativa Rp1-like 1). Analysis of expression of a super green fluorescent protein (sGFP) fusion gene revealed that the OsRP1L1 protein localizes to the nucleolus. Expression patterns suggested that OsRP1L1 was involved in responses to several plant growth regulators (PGRs) and environmental stresses. To explore its function in bacterial blight (BB) resistance, OsRP1L1 was cloned and overexpressed in a susceptible japonica rice cultivar. Overexpression of OsRP1L1 moderately elevated the resistance of plants to Xanthomonas oryzae pv. oryzae strains PXO86 and PXO341. Transcriptome analysis showed that plants overexpressing OsRP1L1 had small changes in their transcriptome, with 26 genes showing statistically significant changes of more than two-fold. The most up-regulated transcripts—a DIN1-like gene and a terpene synthase (TPS) gene—were up-regulated 7.9- and 7.6-fold, respectively. It was also noted that seven of the up-regulated genes have also reportedly been induced by overexpression of OsbZIP46, which has been identified recently as a player in ABA sensing. All these findings suggest that OsRP1L1 plays an important role in rice BB resistance, and may be useful as a genetic resource for engineering disease resistance in plants.

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Acknowledgments

This work was supported by Chinese New Genetically Modified Organism Varieties Programme (2011ZX08009-003-001), National Natural Science Foundation of China for young scholars (30900263, 30900098, 31101208, 31201482), Zhejiang Provincial Foundation for Natural Science (LY12C14011, Y3100591), Zhejiang Leading Team of Science and Technology Innovation and the Program for Leading Team of Agricultural Research and Innovation of Ministry of Agriculture, China. We thank Xutao Hong at Zhejiang-California International NanoSystems Institute for advice on microarray data analysis and Professor M. J. Adams, Rothamsted Research, UK for his correction of the English manuscript.

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Correspondence to Jianping Chen.

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Supplementary Table S1

Primers for identification of positive transgenic lines. (DOC 28 kb)

Supplementary Table S2

Primers used for expression analysis of OsRP1L1. (Expression analysis, semiquantitative RT-PCR and qRT-PCR were all performed with these primers, which were designed with Primer Premier 5 Software. The qRT-PCR analyses were performed with SsoFast EvaGreen Supermix). (DOC 28 kb)

Supplementary Table S3

Primers used for expression analysis of PR1 genes. (DOC 40 kb)

Supplementary Table S4

Primer sequences and UPL probes used in quantitative real-time PCR for verification of microarray analysis. (DOC 42 kb)

Supplementary Table S5

Genes (probe sets) showing more than 2-fold enhanced expression in plants overexpressing RP1L1 compared with wild type. (DOC 42 kb)

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Wang, X., Chen, J., Yang, Y. et al. Characterization of a Novel NBS-LRR Gene Involved in Bacterial Blight Resistance in Rice. Plant Mol Biol Rep 31, 649–656 (2013). https://doi.org/10.1007/s11105-012-0537-0

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  • DOI: https://doi.org/10.1007/s11105-012-0537-0

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