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The Negative Regulator OsSDS1 Significantly Reduces Salt and Drought Tolerance in Transgenic Arabidopsis

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Abstract

In this report, we present data on OsSDS1 (Oryza sativa L. salt and drought sensitive gene 1)—an uncharacterized gene isolated from rice Pei’ai 64S (O. sativa L.). Expression of OsSDS1 was strongly up-regulated by a wide spectrum of stresses, including cold, drought, and heat, in different tissues at different developmental stages of rice, as revealed by both microarray and quantitative RT-PCR analyses. Subcellular localization revealed that an OsSDS1: GFP fusion protein was distributed to the nucleus. Expression of OsSDS1 conferred decreased tolerance to salt and drought in Arabidopsis thaliana, accompanied by altered expression of stress-responsive genes and altered K+/Na+ ratio. The results suggest that OsSDS1 may act as a negative regulator of salt and drought tolerance in plants.

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Abbreviations

CaMV:

Cauliflower mosaic virus

GFP:

Green fluorescent protein

Pn:

Net photosynthesis rate

qRT-PCR:

Quantitative real-time RT-PCR

WT:

Wild type

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Acknowledgments

This research was supported by Nitrogen and Phosphorus cycling and manipulation for agro-ecosystems and the Knowledge Innovation program of the Chinese Academy of Sciences (KZCX2-YW-T07) and National Natural Science Foundation of China (31171536).

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Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China

    Guoyun Xu, Mingjuan Li, Mei Huang, Yanchun Cui, Manling Wang & Xinjie Xia

Authors
  1. Guoyun Xu
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  2. Mingjuan Li
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  3. Mei Huang
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  4. Yanchun Cui
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  5. Manling Wang
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  6. Xinjie Xia
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Corresponding author

Correspondence to Xinjie Xia.

Additional information

G. Xu and M. Li contributed equally to this work

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Xu, G., Li, M., Huang, M. et al. The Negative Regulator OsSDS1 Significantly Reduces Salt and Drought Tolerance in Transgenic Arabidopsis. Plant Mol Biol Rep 31, 517–523 (2013). https://doi.org/10.1007/s11105-012-0521-8

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