Abstract
Alternative splicing (AS), i.e., generating multiple mRNA transcripts from a single gene, is known to contribute to proteome diversity and gene regulation in eukaryotes. OsHsfA2a, a gene encoding a heat shock transcription factor (HSF) in rice (Oryza. sativa L. cv. Nipponbare) generates six transcript isoforms (OsHsfA2a-1 to OsHsfA2a-6) by AS. In this study, an OsHsfA2a has been identified and the genomic sequence found to be composed of three exons and two introns. Moreover, stress-responsive and phytohormone-responsive cis-elements in the promoter regions of OsHsfA2a have been identified. Real-time quantitative polymerase chain reaction analysis has revealed that the six isoforms have different tissue-specific expression patterns under normal growth conditions in rice. Interestingly, when treated with ABA (abscisic acid) and various abiotic stresses such as heat shock, simulated drought and high NaCl, these isoforms also show various stress responsive patterns in rice seedlings. These results indicate that AS of OsHsfA2a may play an important role not only in rice growth and development, but also in regulating stress-specific cellular adaptation responses.
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Acknowledgments
We thank I.S. hadad for helpful advice with experiments. This research was supported by “Project 211” of Jilin University, the project of technological guidance from Jilin Provincial Science and Technology Department (201205012) and the basic scientific research project of Jilin University (450060481139).
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Wang, H., Bian, M., Yang, Z. et al. Preliminary Functional Analysis of the Isoforms of OsHsfA2a (Oryza sativa L.) Generated by Alternative Splicing. Plant Mol Biol Rep 31, 38–46 (2013). https://doi.org/10.1007/s11105-012-0471-1
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DOI: https://doi.org/10.1007/s11105-012-0471-1