Abstract
Plant lipoxygenases (LOXs) are functionally diverse class of dioxygenases involved in multiple physiological processes such as plant growth, biotic and abiotic stress responses, and secondary metabolite accumulation. In this paper, two LOX genes, TcLOX1 and TcLOX2, were cloned from Taxus chinensis cells. Multiple alignment of the deduced amino acid sequences with those of other plants demonstrated the putative LH2/PLAT domain, lipoxygenase iron-binding catalytic domain, lipoxygenase_1 and lipoxygenase_2 signature sequences. Phylogenetic analysis suggested that TcLOX1 and TcLOX2 putative proteins are most probably 9-LOXs, and shared the highest identity with the tea plant CsLOX1 and Picea sitchensis LOX genes, respectively. Semiquantitative RT-PCR analysis showed that TcLOX1 was preferentially expressed in stem and root, while TcLOX2 was preferentially expressed in root. Real-time quantitative PCR analysis showed that a strong upregulation of TcLOX1 was observed in response to methyl jasmonate and abscisic acid (ABA), while TcLOX2 was strongly upregulated by ABA. However, TcLOX1 and TcLOX2 were nearly not responding to salicylic acid. These data suggest both TcLOX1 and TcLOX2 play an important role in T. chinensis, and they are required in different physiological processes involved in different plant signals in vivo.
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Abbreviations
- MeJA:
-
Methyl jasmonate
- ABA:
-
Abscisic acid
- SA:
-
Salicylic acid
- RT-PCR:
-
Reverse transcriptase PCR
- Real-time Q-PCR:
-
Real-time quantitative PCR
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Acknowledgments
This work was supported by National Natural Science Foundation of China (Grant 20906036, 20776058 and 201076093), National “11th Five-Year Plan” to Support Science and Technology Project of China (2008BAI63B04), and New Century Talents Support Program by the Ministry of Education of China in 2006.
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Shu-tao Li and Meng Zhang contributed equally to this work.
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Li, St., Zhang, M., Fu, Ch. et al. Molecular Cloning and Characterization of Two 9-Lipoxygenase Genes from Taxus chinensis . Plant Mol Biol Rep 30, 1283–1290 (2012). https://doi.org/10.1007/s11105-012-0439-1
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DOI: https://doi.org/10.1007/s11105-012-0439-1