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Soils naturally suppressive to banana Fusarium wilt disease harbor unique bacterial communities

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Abstract

Aims

Banana Fusarium wilt disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to disease suppressiveness.

Methods

Soil bacteria communities from the two banana orchards with excellent Fusarium disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium wilt disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing.

Result

Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in disease-suppressive soils differed from disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with disease suppression.

Conclusions

Naturally disease suppressive soils to banana Fusarium wilt disease harbor unique bacterial communities.

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Acknowledgments

This research was supported by the National Key Basic Research Program of China (2015CB150506), the National Natural Science Foundation of China (31372142 and 41101231), the Department of Science and Technology of Hainan Province (ZDZX2013023), the Chinese Ministry of Science and Technology (2013AA102802), the Priority Academic Program Development of the Jiangsu Higher Education Institutions (PAPD), the 111 project (B12009), the Agricultural Ministry of China (201103004), National Key Technology R&D Program of the Ministry of Science and Technology (2011BAD11B03) and the Innovative Research Team Development Plan of the Ministry of Education of China (IRT1256).

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    Authors and Affiliations

    1. Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China

      Zongzhuan Shen, Chao Xue, Shutang Zhong, Rong Li & Qirong Shen

    2. Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, College of Agriculture, Hainan University, 570228, Haikou, China

      Yunze Ruan

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    1. Zongzhuan Shen
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    3. Chao Xue
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    Correspondence to Rong Li.

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    Responsible Editor: Jeff R. Powell .

    Zongzhuan Shen and Yunze Ruan contributed equally to this work.

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    Shen, Z., Ruan, Y., Xue, C. et al. Soils naturally suppressive to banana Fusarium wilt disease harbor unique bacterial communities. Plant Soil 393, 21–33 (2015). https://doi.org/10.1007/s11104-015-2474-9

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