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Proteomic characterization of silicon-mediated resistance against Ralstonia solanacearum in tomato

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Abstract

Background and aims

Silicon has an important role in enhancing resistance of plants against multiple environmental stresses including pathogen infection, but the mechanism is still not completely understood. In the present study, the role of Si-mediated resistance to Ralstonia solanacearum in tomato (Solanum lycopersicum) root was explored by a proteomics approach.

Methods

Treatments consisted of pathogen inoculation with or without 2.0 mM Si amendments, and two controls with or without 2.0 mM Si amendments. Proteins from the tomato roots with different treatments were extracted and identified by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass spectrometry (LC-MS/MS).

Results

Fifty-three protein spots were identified at least two-fold differences in abundance on 2-DE maps under R. solanacearum inoculation and/or Si application. Among these proteins, 40 were significantly altered (6 were up-accumulated and 34 were down-accumulated) by R. solanacearum inoculation only. And 26 were altered (16 were increased and 10 were decreased) when Si was added to R. solanacearum-inoculated tomato plants. More than half of the altered proteins (62 %) were associated with energy/metabolism including glycolytic pathway and TCA cycle. Five proteins were grouped into defense-response, of which four were membrane-associated proteins.

Conclusions

These findings provide insights into molecular mechanisms responsible for Si-mediated resistance of tomato against R. solanacearum.

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Abbreviations

2-DE:

Two-dimensional electrophoresis

2-D IEF/SDS–PAGE:

2 dimensional isoelectric focusing/sodium dodecyl sulphate–polyacrylamide gel electrophoresis

TTC:

2,3,5-Triphenyltetrazolium chloride

DMRT:

Duncan multiple range test

IEF:

Isoelectric focusing

Si:

Silicon

R. solanacearum :

Ralstonia solanacearum

IPG:

Immobilized pH gradient

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

MS:

Mass spectrometry

CBB:

Coomassie Brilliant Blue

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Acknowledgments

This study was financially supported by grants from the National Key Basic Research Funds of China (2011CB100400), the National Natural Science Foundation of China (31370456), Doctoral Foundation of the Ministry of Education of China (20124404110007) and the Natural Science Foundation of Guangdong Province (S2012010010331). We would like to thank Professor Shennan Carol and Dr. Muramoto Joji at University of California, Santa Cruz for reading this paper and giving their useful suggestions and comments of writing.

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Authors and Affiliations

  1. Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Yuting Chen, Min Liu, Lei Wang, Weipeng Lin, Xueying Fan & Kunzheng Cai

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  1. Yuting Chen
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  2. Min Liu
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  3. Lei Wang
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Correspondence to Kunzheng Cai.

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Responsible Editor: Yong Chao Liang.

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Chen, Y., Liu, M., Wang, L. et al. Proteomic characterization of silicon-mediated resistance against Ralstonia solanacearum in tomato. Plant Soil 387, 425–440 (2015). https://doi.org/10.1007/s11104-014-2293-4

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