Skip to main content

Advertisement

SpringerLink
Log in

Arbuscular mycorrhizal fungal communities are influenced by agricultural land use and not soil type among the Chernozem great groups of the Canadian Prairies

  • Regular Article
  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Background and aims

The aim of this study was to explore the diversity of arbuscular mycorrhizal (AM) fungi in agroecosystems of the Canadian Prairies and determine whether soil type and land use impact the diversity and composition of these important communities.

Methods

We used two separate field surveys and methods to address these questions. The first survey involved collecting soil samples from five different soil types and spores were morphologically identified from trap cultures established using these soil samples. The second survey involved collecting soil samples from two different land use types across the four major Chernozem great groups to characterize the AM fungal communities using 454 GS FLX pyrosequencing of the small subunit rDNA region.

Results

The first survey found that Vertisolic soil had a significantly higher AM spore richness compared to the Chernozem soils. Both surveys revealed no effect of soil type on the diversity and composition of AM fungal communities among the Chernozem great groups. The two different methods used in this study revealed differences in the proportional representation of certain AM fungal taxa. Land use had a strong impact on the AM fungal communities in the Canadian Prairies as roadsides harboured a more diverse and compositionally different communities compared to annually cropped fields.

Conclusions

Overall, soil type appears to have little to no effect on AM fungal communities among the Chernozem great groups in the Canadian Prairies, and land use practices impact the diversity and composition of these communities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig 1
Fig 2
Fig 3

Similar content being viewed by others

References

  • Auge RM (2001) Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11:3–42

    Article  Google Scholar 

  • Bainard LD, Koch AM, Gordon AM, Newmaster SG, Thevathasan NV, Klironomos JN (2011) Influence of trees on the spatial structure of arbuscular mycorrhizal communities in a temperate tree-based intercropping system. Agr Ecosyst Environ 144:13–20

    Article  Google Scholar 

  • Bainard LD, Bainard JD, Hamel C, Gan Y (2014) Spatial and temporal structuring of arbuscular mycorrhizal communities is differentially influenced by abiotic factors and host crop in a semi-arid prairie agroecosystem. FEMS Microbiol Ecol. doi:10.1111/1574-6941.12300

    PubMed  Google Scholar 

  • Boyetchko SM, Tewari JP (1993) Occurrence of vesicular-arbuscular mycorrhizal fungi in Alberta, Canada. Z Naturforsch Sect C J Biosci 48:923–929

    CAS  Google Scholar 

  • Dai M, Hamel C, St. Arnaud M, He Y, Grant C, Lupwayi N, Janzen H, Malhi SS, Yang X, Zhou Z (2012) Arbuscular mycorrhizal fungi assemblages in Chernozem great groups revealed by massively parallel pyrosequencing. Can J Microbiol 58:81–92

    Article  CAS  PubMed  Google Scholar 

  • Dai M, Bainard LD, Hamel C, Gan Y, Lynch D (2013) Impact of land use on arbuscular mycorrhizal fungal communities in rural Canada. Appl Environ Microbiol 79:6719–6729

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Daniels BA, Skipper HD (1982) Methods for the recovery and quantitative estimation of propagules from soil. In: Schenck N (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St Paul, pp 29–35

    Google Scholar 

  • de Carcer DA, Denman SE, McSweeney C, Morrison M (2011) Evaluation of subsampling-based normalization strategies for tagged high-throughput sequencing data sets from gut microbiomes. Appl Environ Microbiol 77:8795–8798

    Article  PubMed  Google Scholar 

  • Dumbrell AJ, Nelson M, Helgason T, Dytham C, Fitter AH (2010) Relative roles of niche and neutral processes in structuring a soil microbial community. ISME J 4:337–345

    Article  PubMed  Google Scholar 

  • Fitter AH, Helgason T, Hodge A (2011) Nutritional exchanges in the arbuscular mycorrhizal symbiosis: implications for sustainable agriculture. Fungal Biol Rev 25:68–72

    Article  Google Scholar 

  • Garg N, Chandel S (2011) Effect of mycorrhizal inoculation on growth, nitrogen fixation, and nutrient uptake in Cicer arietinum (L.) under salt stress. Turk J Agric For 35:205–214

    CAS  Google Scholar 

  • Gerdemann JW, Trappe JM (1974) The endogonaceae in the Pacific Northwest. Mycologia Mem 5:1–76

    Google Scholar 

  • Gianinazzi S, Gollotte A, Binet MN, van Tuinen D, Redecker D, Wipf D (2010) Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza 20:519–530

    Article  PubMed  Google Scholar 

  • Gosling P, Mead A, Proctor M, Hammond JP, Bending GD (2013) Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient. New Phytol 198:546–556

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hijri I, Sykorova Z, Oehl F et al (2006) Communities of arbuscular mycorrhizal fungi in arable soils are not necessarily low in diversity. Mol Ecol 15:2277–2289

    Article  CAS  PubMed  Google Scholar 

  • Jansa J, Mozafar A, Anken T et al (2002) Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza 12:225–234

    Article  CAS  PubMed  Google Scholar 

  • Johnson NC, Zak DR, Tilman D, Pfleger FL (1991) Dynamics of vesicular-arbuscular mycorrhizae during old field succession. Oecologia 86:349–358

    Article  Google Scholar 

  • Jumpponen A, Trowbridge J, Mandyam K, Johnson L (2005) Nitrogen enrichment causes minimal changes in arbuscular mycorrhizal colonization but shifts community composition – evidence from rDNA data. Biol Fert Soils 41:217–224

    Article  CAS  Google Scholar 

  • Landis FC, Gargas A, Givnish TJ (2004) Relationships among arbuscular mycorrhizal fungi, vascular plants and environmental conditions in oak savannas. New Phytol 164:493–504

    Article  Google Scholar 

  • Lekberg Y, Koide RT, Rohr JR, Aldrich-Wolfe L, Morton JB (2007) Role of niche restrictions and dispersal in the composition of arbuscular mycorrhizal fungal communities. J Ecol 95:95–105

    Article  Google Scholar 

  • Lekberg Y, Meadow J, Rohr JR, Redecker D, Zabinski CA (2011) Importance of dispersal and thermal environment for mycorrhizal communities: lessons from Yellowstone National Park. Ecology 92:1292–1302

    Article  PubMed  Google Scholar 

  • Lin X, Feng Y, Zhang H et al (2012) Long-term balanced fertilization decreases arbuscular mycorrhizal fungal diversity in an arable soil in North China revealed by 454 pyrosequencing. Envir Sci Tech 46:5764–5771

    Article  CAS  Google Scholar 

  • Lumini E, Orgiazzi A, Borriello R, Bonfante P, Biancotto V (2010) Disclosing arbuscular mycorrhizal fungal biodiversity in soil through a land-use gradient using a pyrosequencing approach. Environ Microbiol 12:2165–2179

    CAS  PubMed  Google Scholar 

  • Ma WK, Siciliano SD, Germida JJ (2005) A PCR-DGGE method for detecting arbuscular mycorrhizal fungi in cultivated soils. Soil Biol Biochem 37:1589–1597

    Article  CAS  Google Scholar 

  • Manning G, Fuller LG, Eilers RG, Florinsky I (2001) Topographic influence on the variability of soil properties within an undulating Manitoba landscape. Can J Soil Sci 81:439–447

    Article  CAS  Google Scholar 

  • Minchin PR (1987) An evaluation of the relative robustness of techniques for ecological ordination. Vegetatio 69:89–107

    Article  Google Scholar 

  • Moebius-Clune DJ, Moebius-Clune BN, van Es HM, Pawlowska TE (2013) Arbuscular mycorrhizal fungi associated with a single agronomic plant host across the landscape: community differentiation along a soil textural gradient. Soil Biol Biochem 64:191–199

    Article  CAS  Google Scholar 

  • Morris EK, Buscot F, Herbst C, Meiners T, Obermaier E, Waschke NW, Wubet T, Rillig MC (2013) Land use and hot neighbor identity effects on arbuscular mycorrhizal fungal community composition in focal plant rhizosphere. Biodivers Conserv 22:2193–2205

    Article  Google Scholar 

  • Newsham KK, Fitter AH, Watkinson AR (1995) Arbuscular mycorrhiza protect an annual grass from root pathogenic fungi in the field. J Ecol 83:991–1000

    Article  Google Scholar 

  • Oehl F, Sieverding E, Ineichen K, Mader P, Boller T, Wiemken A (2003) Impact of land use intensity on the species diversity or arbuscular mycorrhizal fungi in agroecosystems of central Europe. Appl Environ Microbiol 69:2816–2824

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Oehl F, Laczko E, Bogenrieder A, Stahr K, Bosch R, van der Heijden M, Sieverding E (2010) Soil type and land use intensity determine the composition of arbuscular mycorrhizal fungal communities. Soil Biol Biochem 42:724–738

    Article  CAS  Google Scholar 

  • Oehl F, Silva GA, Goto BT, Sieverding E (2011) Glomeromycota: three new genera and glomoid species reorganized. Mycotaxon 116:75–120

    Article  Google Scholar 

  • Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens HH, Wagner H (2013) vegan: Community Ecology Package. R Package Version 2.0-10. http://CRAN.R-project.org/package=vegan

  • Polz MF, Cavanaugh CM (1998) Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol 64:3724–3730

    CAS  PubMed Central  PubMed  Google Scholar 

  • Redecker D, Schussler A, Stockinger H, Sturmer SL, Morton JB, Walker C (2013) An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota). Mycorrhiza 23:515–531

    Article  PubMed  Google Scholar 

  • Rillig MC, Mummey DL (2006) Mycorrhizas and soil structure. New Phytol 171:41–53

    Article  CAS  PubMed  Google Scholar 

  • Sato K, Suyama Y, Saito M, Sugawara K (2005) A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction-denature gradient gel electrophoresis. Jap Soc Grassland Sci 51:179–181

    Article  CAS  Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Academic, London

    Google Scholar 

  • Soil Classification Work Group (1998) The Canadian system of soil classification, 3rd edn. Agriculture and Agri-Food Canada Publication, Ottawa, Available from http://sis.agr.gc.ca/cansis/taxa/cssc3/intro.html

    Google Scholar 

  • Stevenson FJ, Cole MA (1999) The carbon cycle. In: Cycles of Soils. John Wiley & Sons Inc. New York p. 7–10

  • Talukdar NC, Germida JJ (1993) Occurrence and isolation of vesicular-arbuscular mycorrhizae in cropped field soils of Saskatchewan, Canada. Can J Microbiol 39:567–575

    Article  Google Scholar 

  • Verbruggen E, van der Heijden MGA, Weedon JT, Kowalchuck GA, Roling WFM (2012) Community assembly, species richness and nestedness of arbuscular mycorrhizal fungi in agricultural soils. Mol Ecol 12:2341–2353

    Article  Google Scholar 

  • Wang YY, Vestberg M, Walker C, Hurme T, Zhang X, Lindstrom K (2008) Diversity and infectivity of arbuscular mycorrhizal fungi in agricultural soils of the Sichuan Province of mainland China. Mycorrhiza 18:59–68

    Article  PubMed  Google Scholar 

  • Wheeler B (2010) lmperm: Permutation tests for linear models. R Package Version 1.1-2. http://cran.r-project.org/web/packages/lmPerm

  • Yang C, Hamel C, Schellenberg MP, Perez JC, Berbara RL (2010) Diversity and functionality of arbuscular mycorrhizal fungi in three plant communities in semiarid Grasslands National Park, Canada. Microb Ecol 59:724–733

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Peer-review Granting Program of Agriculture and Agri-Food Canada and by Project CRN-II-14 of the Inter American Institute for Global Change Research.

Author information

Author notes

  1. Min Sheng & Warren Eilers

    Present address: College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China

  2. Mulan Dai

    Present address: Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, People’s Republic of China

Authors and Affiliations

  1. Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, Swift Current, SK, S9H 3X2, Canada

    Luke D. Bainard, Mulan Dai, Min Sheng & Chantal Hamel

  2. Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, Calabazar, Boyeros, CP 11900, La Habana, Cuba

    Eduardo Furrazola Gomez & Yamir Torres-Arias

  3. Retired, Saskatoon Research Centre, Agriculture and Agri-Food Canada (AAFC), 107 Science Place, Saskatoon, SK, S7N 0X2, Canada

    Warren Eilers

  4. Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Jillian D. Bainard

Authors
  1. Luke D. Bainard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mulan Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eduardo Furrazola Gomez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yamir Torres-Arias
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jillian D. Bainard
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Min Sheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Warren Eilers
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chantal Hamel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luke D. Bainard.

Additional information

Responsible Editor: Jeff R. Powell.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PPTX 909 kb)

ESM 2

(PPT 83 kb)

Table S1

Soil chemical characteristics of soil groups sampled in experiment #1 (DOC 30 kb)

Table S2

Soil characteristics of Chernozem soil groups sampled in experiment #2 (DOC 38 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bainard, L.D., Dai, M., Gomez, E.F. et al. Arbuscular mycorrhizal fungal communities are influenced by agricultural land use and not soil type among the Chernozem great groups of the Canadian Prairies. Plant Soil 387, 351–362 (2015). https://doi.org/10.1007/s11104-014-2288-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11104-014-2288-1

Keywords

Search

Navigation