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Integration of metal chemical forms and subcellular partitioning to understand metal toxicity in two lettuce (Lactuca sativa L.) cultivars

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Abstract

Aims

Metal chemical forms and subcellular partitioning model (SPM) in organisms can provide valuable insights into metal toxicity.

Methods

Two cultivars of lettuce (Lactuca sativa L.) were grown in Cd and Cu contaminated soils and chemical forms and subcellular distribution of Cd and Cu within the lettuce shoots were determined.

Results

Examination of the inhibition of superoxide dismutase (SOD) and catalase (CAT) activities, as well as the production of H2O2 showed that Lactuca sativa L. var. longifolia is more sensitive to metal-stress than is Lactuca sativa L. var. crispa. In L. crispa, the majority of accumulated Cd was in the pectate- and protein-integrated forms (53.7–62.9 %), while in L. longifolia, a higher proportion of the Cd was in the water soluble forms (33.0–39.2 %) and in the organelles fraction – these forms being potentially associated with toxicity. The chemically-based chemical form approach agreed closely with independent biologically-based SPM, as demonstrated by their significant linear relationships.

Conclusions

This study provides a first step towards the integration of chemical form approach and SPM into a common mechanistic framework, which is important for predicting the likelihood of toxic effects of metals in the environment of interest.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21077111; 21105007). We thanks Dr. Peter Kopittke and Dr. Peng Wang from the University of Queensland, for their careful English revision and important suggestions to the final version of this paper.

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Authors and Affiliations

  1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, People’s Republic of China

    Cheng-Cheng Li, Fei Dang, Long Cang, Chui-Fan Zhou & Dong-Mei Zhou

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Cheng-Cheng Li

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  1. Cheng-Cheng Li
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  2. Fei Dang
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  3. Long Cang
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  4. Chui-Fan Zhou
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Correspondence to Dong-Mei Zhou.

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Responsible Editor: Juan Barcelo.

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Li, CC., Dang, F., Cang, L. et al. Integration of metal chemical forms and subcellular partitioning to understand metal toxicity in two lettuce (Lactuca sativa L.) cultivars. Plant Soil 384, 201–212 (2014). https://doi.org/10.1007/s11104-014-2194-6

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  • DOI: https://doi.org/10.1007/s11104-014-2194-6

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