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Growth response of crops to soil microbial communities from conventional monocropping and tree-based intercropping systems

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A Commentary to this article was published on 08 December 2012

Abstract

Background and aims

Recent studies have shown that tree-based intercropping (TBI) systems support a more diverse soil microbial community compared to conventional agricultural systems. However, it is unclear whether differences in soil microbial diversity between these two agricultural systems have a functional effect on crop growth.

Methods

In this study, we used a series of greenhouse experiments to test whether crops respond differently to the total soil microbial community (Experiment 1) and to arbuscular mycorrhizal (AM) fungal communities alone (Experiment 2) from conventionally monocropped (CM) and TBI systems.

Results

The crops had a similar growth response to the total soil microbial communities from both cropping systems. However, when compared to sterilized controls, barley (Hordeum vulgare) and canola (Brassica napus) exhibited a negative growth response to the total soil microbial communities, while soybean (Glycine max) was unaffected. During the AM fungal establishment phase of the second experiment, ‘nurse’ plants had a strong positive growth response to AM fungal inoculation, and significantly higher biomass when inoculated with AM fungi from the CM system compared to the TBI system. Soybean was the only crop species to exhibit a significant positive growth response to AM fungal inoculation. Similar to the total soil microbial communities, AM fungi from the two cropping systems did not differ in their effect on crop growth.

Conclusion

Overall, AM fungi from both cropping systems had a positive effect on the growth of plants that formed a functional symbiosis. However, the results from these experiments suggest that negative effects of non-AM fungal microbes are stronger than the beneficial effects of AM fungi from these cropping systems.

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References

  • Altieri MA (1999) The ecological role of biodiversity in agroecosystems. Agr Ecosyst Environ 74:19–31

    Article  Google Scholar 

  • Bainard LD, Koch AM, Gordon AM, Newmaster SG, Thevathasan NV, Klironomos JN (2011) Influence of trees on the spatial structure of arbuscular mycorrhizal communities in a temperate tree-based intercropping system. Agr Ecosyst Environ 144:13–20

    Article  Google Scholar 

  • Bainard LD, Koch AM, Gordon AM, Klironomos JN (2012) Temporal and compositional differences of arbuscular mycorrhizal fungal communities in conventional monocropping and tree-based intercropping systems. Soil Biol Biochem 45:172–180

    Article  CAS  Google Scholar 

  • Beare MH, Coleman DC, Crossley DA, Hendrix PF, Odum EP (1995) A hierarchical approach to evaluating the significance of soil biodiversity to biogeochemical cycling. Plant Soil 170:5–22

    Article  CAS  Google Scholar 

  • Brundrett M (1994) Clearing and staining mycorrhizal roots. In: Brundrett M, Melville L, Peterson L (eds) Practical methods in mycorrhizal research. Mycologue Publications, Waterloo, pp 42–46

    Google Scholar 

  • Chalk PM, de F Souza R, Urquiaga S, Alves BJR, Boddey RM (2006) The role of arbuscular mycorrhiza in legume symbiotic performance. Soil Biol Biochem 38:2944–2951

    Article  CAS  Google Scholar 

  • Clarke C, Mosse B (1981) Plant growth responses to vesicular-arbuscular mycorrhiza. Part XII: Field inoculation responses of barley at two soil P levels. New Phytol 87:695–703

    Article  CAS  Google Scholar 

  • Daniels BA, Skipper HD (1982) Methods for the recovery and quantitative estimation of propagules from soil. In: Schenck NC (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St. Paul, pp 29–35

    Google Scholar 

  • Franklin RB, Mills AL (2009) Importance of spatially structured environmental heterogeneity in controlling microbial community composition at small spatial scales in an agricultural field. Soil Biol Biochem 41:1833–1840

    Article  CAS  Google Scholar 

  • Grace EJ, Cotsaftis O, Tester M, Smith FA, Smith SE (2009) Arbuscular mycorrhizal inhibition of growth in barley cannot be attributed to extent of colonization, fungal phosphorus uptake or effects on expression of plant phosphate transporter genes. New Phytol 181:938–949

    Article  PubMed  CAS  Google Scholar 

  • Hart MM, Trevors JT (2005) Microbe management: application of mycorrhizal fungi in sustainable agriculture. Front Ecol Environ 3:533–539

    Article  Google Scholar 

  • Jakobsen I, Jensen A (1981) Influence of vesicular-arbuscular mycorrhiza and straw mulch on growth of barley. Plant Soil 62:157–161

    Article  Google Scholar 

  • Jansa J, Smith FA, Smith SE (2008) Are there benefits of simultaneous root colonization by different arbuscular mycorrhizal fungi? New Phytol 177:779–789

    Article  PubMed  CAS  Google Scholar 

  • Jensen A (1982) Influence of four vesicular-arbuscular mycorrhizal fungi on nutrient uptake and growth in barley (Hordeum vulgare). New Phytol 90:45–50

    Article  CAS  Google Scholar 

  • Jensen A (1984) Responses of barley, pea, and maize to inoculation with different vesicular-arbuscular mycorrhizal fungi in irradiated soil. Plant Soil 78:315–323

    Article  Google Scholar 

  • Johnson NC (1993) Can fertilization of soil select less mutualistic mycorrhizae. Ecol Appl 3:749–757

    Article  Google Scholar 

  • Khaliq A, Sanders FE (2000) Effects of vesicular-arbuscular mycorrhizal inoculation on the yield and phosphorus uptake of field-grown barley. Soil Biol Biochem 32:1691–1696

    Article  CAS  Google Scholar 

  • Kibblewhite MG, Ritz K, Swift MJ (2008) Soil health in agricultural systems. Phil Trans R Soc B 363:685–701

    Article  PubMed  CAS  Google Scholar 

  • Lacombe S, Bradley RL, Hamel C, Beaulieu C (2009) Do tree-based intercropping systems increase the diversity and stability of soil microbial communities? Agr Ecosyst Environ 131:25–31

    Article  Google Scholar 

  • Maherali H, Klironomos JN (2007) Influence of phylogeny on fungal community assembly and ecosystem functioning. Science 316:1746–1748

    Article  PubMed  CAS  Google Scholar 

  • Marschner P, Timonen S (2005) Interactions between plant species and mycorrhizal colonization on the bacterial community composition in the rhizosphere. Appl Soil Ecol 28:23–36

    Article  Google Scholar 

  • Matson PA, Parton WJ, Power AG, Swift MJ (1997) Agricultural intensification and ecosystem properties. Science 277:504–509

    Article  PubMed  CAS  Google Scholar 

  • McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol 115:495–501

    Article  Google Scholar 

  • McNamara NP, Black HIJ, Beresford NA, Parekh NR (2003) Effects of acute gamma irradiation on chemical, physical and biological properties of soil. Appl Soil Ecol 24:117–132

    Article  Google Scholar 

  • Meghvansi MK, Prasad K, Harwani D, Mahna SK (2008) Response of soybean cultivars toward inoculation with three arbuscular mycorrhizal fungi and Bradyrhizobium japonicum in the alluvial soil. Eur J Soil Biol 44:316–323

    Article  CAS  Google Scholar 

  • Mungai NW, Motavalli PP, Kremer RJ, Nelson KA (2005) Spatial variation of soil enzyme activities and microbial diversity in temperate alley cropping systems. Biol Fert Soils 42:129–136

    Article  Google Scholar 

  • Oehl F, Sieverding E, Ineichen K, Mader P, Boller T, Wiemken A (2003) Impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of central Europe. Appl Environ Microbiol 69:2816–2824

    Article  PubMed  CAS  Google Scholar 

  • Oelbermann M, Voroney RP (2007) Carbon and nitrogen in a temperate agroforestry system: using stable isotopes as a tool to understand soil dynamics. Ecol Eng 29:342–349

    Article  Google Scholar 

  • Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular 939, US Government Printing Office, Washington DC

  • Plenchette C, Clermont-Dauphin C, Meynard JM, Fortin JA (2005) Managing arbuscular mycorrhizal fungi in cropping systems. Can J Plant Sci 85:31–40

    Article  Google Scholar 

  • Powell CL (1981) Inoculation of barley with efficient mycorrhizal fungi stimulates seed yield. Plant Soil 59:487–489

    Article  CAS  Google Scholar 

  • Rillig MC, Mummey DL (2006) Mycorrhizas and soil structure. New Phytol 171:41–53

    Article  PubMed  CAS  Google Scholar 

  • Seiter S, Ingham ER, William RD (1999) Dynamics of soil fungal and bacterial biomass in a temperate climate alley cropping system. Appl Soil Ecol 12:139–147

    Article  Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic, New York

    Google Scholar 

  • Solaiman Z, Marschner P, Wang D, Rengel Z (2007) Growth, P uptake and rhizosphere properties of wheat and canola genotypes in an alkaline soil with low P availability. Biol Fert Soils 44:143–153

    Article  CAS  Google Scholar 

  • Thevathasan NV, Gordon AM (1997) Poplar leaf biomass distribution and nitrogen dynamics in a poplar-barley intercropped system in southern Ontario, Canada. Agroforest Syst 37:79–90

    Article  Google Scholar 

  • van der Heijden MGA, Klironomos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A, Sanders IR (1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396:69–72

    Article  Google Scholar 

  • van der Heijden MGA, Streitwolf-Engel R, Riedl R, Siegrist S, Neudecker A, Ineichen K, Boller T, Wiemken A, Sanders IR (2006) The mycorrhizal contribution to plant productivity, plant nutrition and soil structure in experimental grassland. New Phytol 172:739–752

    Article  PubMed  Google Scholar 

  • van der Heijden MGA, Bardgett RD, van Straalen NM (2008) The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecol Lett 11:296–310

    Article  PubMed  Google Scholar 

  • Vogelsang KM, Reynolds HL, Bever JD (2006) Mycorrhizal fungal identity and richness determine the diversity and productivity of a tallgrass prairie system. New Phytol 172:554–562

    Article  PubMed  Google Scholar 

  • Wagg C, Jansa J, Stadler M, Schmid B, van der Heijden MGA (2011a) Mycorrhizal fungal identity and diversity relaxes plant-plant competition. Ecology 92:1303–1313

    Article  PubMed  Google Scholar 

  • Wagg C, Jansa J, Schmid B, van der Heijden MGA (2011b) Belowground biodiversity effects of plant symbionts support aboveground productivity. Ecol Lett 14:1001–1009

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

The authors acknowledge financial support from a Natural Sciences and Engineering Research Council Strategic Grant, Swiss National Science Foundation grant, and Post-Graduate Scholarships from the Natural Sciences and Engineering Research Council of Canada and the Province of Ontario.

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Correspondence to L. D. Bainard.

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Responsible Editor: Hans Lambers.

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Bainard, L.D., Koch, A.M., Gordon, A.M. et al. Growth response of crops to soil microbial communities from conventional monocropping and tree-based intercropping systems. Plant Soil 363, 345–356 (2013). https://doi.org/10.1007/s11104-012-1321-5

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