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Pre-treatment of salicylic acid enhances resistance of soybean seedlings to Fusarium solani

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Pre-treatment of soybean seedlings with 200 μM salicylic acid before fungal inoculation significantly alleviated disease resistance in soybean seedlings against Fusarium solani infection.

Abstract

Sudden death syndrome of soybean is largely caused by Fusarium solani (F. solani). Salicylic acid (SA) has been reported to induce resistance in plants against many pathogens. However, the effect of exogenous SA application on F. solani infection of soybean is less reported. This study investigated the effect of foliar application of SA on soybean seedlings before F. solani infection. Seedlings were sprayed with 200 µM SA and inoculated with F. solani after 24 h of last SA application. After 3 days post-inoculation, seedlings treated with 200 µM SA showed significantly fewer disease symptoms with increased endogenous SA level, SA marker genes expression and antioxidant activities in the SA-treated seedlings more than the untreated control seedlings. Furthermore, the decrease in hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels was observed in the SA-treated plants as compared to the untreated plants. Analysis of the effect of SA application on F. solani showed that the mycelia growth of F. solani was not affected by SA treatment. Further investigation in this study revealed a decreased in F. solani biomass content in the SA treated seedlings. Results from the present study show that pre-treatment of 200 µM SA can induce resistance of soybean seedlings against F. solani infection.

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Acknowledgements

The work was supported by National Natural Science Foundation of China (31871552, 31671445) and the Science & Technology Department of Sichuan Province (2018HH0108).

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Authors and Affiliations

  1. College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China

    George Bawa, Lingyang Feng, Li Yan, Yongli Du, Jing Shang, Xin Sun, Xiaochun Wang, Liang Yu, Chunyan Liu, Wenyu Yang & Junbo Du

  2. Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu, 611130, China

    George Bawa, Lingyang Feng, Li Yan, Yongli Du, Jing Shang, Xin Sun, Xiaochun Wang, Liang Yu, Chunyan Liu, Wenyu Yang & Junbo Du

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  1. George Bawa
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Contributions

JD conceived and designed the research. GB, LF, LY, YD, JS, XS, XW, LY, CL and WY performed the experiments and analyzed the data. GB and JD wrote the manuscript.

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Correspondence to Junbo Du.

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Bawa, G., Feng, L., Yan, L. et al. Pre-treatment of salicylic acid enhances resistance of soybean seedlings to Fusarium solani. Plant Mol Biol 101, 315–323 (2019). https://doi.org/10.1007/s11103-019-00906-x

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