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In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts

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Abstract

In plant mitochondria, the 5′ ends of many transcripts are generated post-transcriptionally. We show that the pentatricopeptide repeat (PPR) protein RNA PROCESSING FACTOR 4 (RPF4) supports the generation of extra 5′ ends of ccmB transcripts in Landsberg erecta (Ler) and a number of other Arabidopsis thaliana ecotypes. RPF4 was identified in Ler applying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which generates the Ler-type extra ccmB 5′ termini only after the introduction of the RPF4 allele from Ler. Studies with chimeric RPF4 proteins composed of various parts of the RPF4 proteins from Ler and Col identified differences in the N-terminal and central PPR motifs that explain ecotype-specific variations in ccmB processing. These results fit well with binding site predictions in ccmB transcripts based on the known determinants of nucleotide base recognition by PPR motifs.

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Acknowledgements

We are very grateful to Conny Guha and Uli Tengler for excellent technical assistance. This work was supported by DFG Grant Bi 590/10-2.

Author contributions

KS, CJ, SS, C.CdF-S and IS performed experiments and/or analyzed the data; SB conceived the project, designed and supervised experiments and wrote the article with contributions of all the authors.

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Authors and Affiliations

  1. Institut Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany

    Katrin Stoll, Christian Jonietz, Sarah Schleicher & Stefan Binder

  2. Australian Research Council 40 Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, 6009, Australia

    Catherine Colas des Francs-Small & Ian Small

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  1. Katrin Stoll
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  2. Christian Jonietz
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  4. Catherine Colas des Francs-Small
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Correspondence to Stefan Binder.

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Stoll, K., Jonietz, C., Schleicher, S. et al. In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts. Plant Mol Biol 93, 659–668 (2017). https://doi.org/10.1007/s11103-017-0591-y

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