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Identification of two additional members of the tRNA isopentenyltransferase family in Physcomitrella patens

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Abstract

The Physcomitrella patens genome has seven genes apparently coding for the isopentenyltransferase type of tRNA-modifying enzyme, while other organisms have one or two. The predicted sequences have parts that differ significantly from other isopentenyltransferases. Only one of the seven (PpIPT1) has earlier been shown to be expressed. We now report expression of two more, PpIPT4 and PpIPT5. The cloned genes were able to functionally complement a yeast mutant lacking tRNA isopentenyltransferase. Sequencing showed they are related to the earlier studied PpIPT1. The sequences of the three differ mainly from each other in a tRNA-binding area and the 5′-end subcellular targeting motif area. This indicates that, after arising through gene duplication, they have evolved to enable partly different functions.

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Abbreviations

IPT:

Isopentenyltransferase

tRNA-IPT:

tRNA isopentenyltransferase

ATP/ADP-IPT:

Adenylate isopentenyltransferase

PpIPT:

Physcomitrella patens isopentenyltransferase

i6A:

N 6-(Δ2-isopentenyl)adenosine, isopentenyladenosine

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Acknowledgments

We thank Mattias Thelander for teaching the culturing of moss, and Hans Ronne for the Uppsala K-1 strain. Anita K. Hopper is thanked for the yeast lines. Grzegorz Furman is thanked for contributing to setting up the tRNA nucleoside analysis.

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  1. Gunvant Patil

    Present address: Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri, Columbia, MO, USA

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  1. Department of Plant Biology and Forest Genetics and Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Gunvant Patil & Bjorn Nicander

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  1. Gunvant Patil
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  2. Bjorn Nicander
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Correspondence to Bjorn Nicander.

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Patil, G., Nicander, B. Identification of two additional members of the tRNA isopentenyltransferase family in Physcomitrella patens . Plant Mol Biol 82, 417–426 (2013). https://doi.org/10.1007/s11103-013-0072-x

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