Plant Molecular Biology

, Volume 81, Issue 6, pp 565–576

The Arabidopsis thaliana ortholog of a purported maize cholinesterase gene encodes a GDSL-lipase

  • Mrinalini Muralidharan
  • Kristina Buss
  • Katherine E. Larrimore
  • Nicholas A. Segerson
  • Latha Kannan
  • Tsafrir S. Mor
Article

DOI: 10.1007/s11103-013-0021-8

Cite this article as:
Muralidharan, M., Buss, K., Larrimore, K.E. et al. Plant Mol Biol (2013) 81: 565. doi:10.1007/s11103-013-0021-8

Abstract

Acetylcholinesterase is an enzyme that is intimately associated with regulation of synaptic transmission in the cholinergic nervous system and in neuromuscular junctions of animals. However the presence of cholinesterase activity has been described also in non-metazoan organisms such as slime molds, fungi and plants. More recently, a gene purportedly encoding for acetylcholinesterase was cloned from maize. We have cloned the Arabidopsis thaliana homolog of the Zea mays gene, At3g26430, and studied its biochemical properties. Our results indicate that the protein encoded by the gene exhibited lipase activity with preference to long chain substrates but did not hydrolyze choline esters. The At3g26430 protein belongs to the SGNH clan of serine hydrolases, and more specifically to the GDS(L) lipase family.

Keywords

CholinesteraseGDS(L)lipaseSerine hydrolase

Supplementary material

11103_2013_21_MOESM1_ESM.xls (288 kb)
Supplementary material 1 (XLS 288 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Mrinalini Muralidharan
    • 1
  • Kristina Buss
    • 1
  • Katherine E. Larrimore
    • 1
  • Nicholas A. Segerson
    • 1
  • Latha Kannan
    • 1
  • Tsafrir S. Mor
    • 1
  1. 1.School of Life Sciences and The Biodesign InstituteArizona State UniversityTempeUSA