Plant Molecular Biology

, Volume 61, Issue 3, pp 383–397

Disease Resistance in Plants that Carry a Feedback-regulated Yeast Poly(A) Binding Protein Gene

  • Balasubrahmanyam Addepalli
  • Ruqiang Xu
  • Tomal Dattaroy
  • Baochun Li
  • W. Troy Bass
  • Qingshun Q. Li
  • Arthur G. Hunt
Article

DOI: 10.1007/s11103-006-0019-6

Cite this article as:
Addepalli, B., Xu, R., Dattaroy, T. et al. Plant Mol Biol (2006) 61: 383. doi:10.1007/s11103-006-0019-6

Abstract

It has been reported that the expression of the yeast poly(A) binding protein gene (PAB1) in plants leads to an induction of disease resistance responses, accompanied by alterations in the growth habit of the plant (Li et al. Plant Mol. Biol. (2000) 42 335). To capitalize on this observation, a feedback-regulated PAB1 gene was assembled and introduced into tobacco and Arabidopsis. The regulation entailed the linking of the expression of the PAB1 gene to control by the lac repressor, and by linking lac repressor expression to the disease resistance state of the plant, such that the induction of systemic defense responses by accumulation of the yeast poly(A) binding protein would turn off the expression of the PAB1 gene. Plants containing this system showed elevated and/or constitutive expression of disease-associated genes and significant resistance to otherwise pathogenic organisms. As well, they displayed a nearly normal growth habit under laboratory and greenhouse settings. These studies indicate that the expression of cytotoxic genes (such as the PAB1 gene) in plants can be controlled so that enhanced disease resistance can be achieved without significantly affecting plant growth and development.

Keywords

constitutive PR gene expressiondisease resistancefeedback regulationlac repressoryeast poly(A) binding protein

Copyright information

© Springer 2006

Authors and Affiliations

  • Balasubrahmanyam Addepalli
    • 1
  • Ruqiang Xu
    • 2
  • Tomal Dattaroy
    • 1
    • 4
  • Baochun Li
    • 3
  • W. Troy Bass
    • 3
  • Qingshun Q. Li
    • 2
  • Arthur G. Hunt
    • 1
  1. 1.Department of Plant and Soil SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Department of BotanyMiami UniversityOxfordUSA
  3. 3.Kentucky Tobacco Research and Development CenterUniversity of KentuckyLexingtonUSA
  4. 4.Metahelix Life Sciences Pvt. LtdBangaloreIndia