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Analysis of betalains from fruits of Opuntia species

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Abstract

Betalains are of great taxonomic significance in higher plants and occur only in 10 families of the order Caryophyllales (Centrospermae). They are water-soluble nitrogenous pigments. They can be divided into two major structural groups, the red to red-violet betacyanins and the yellow betaxanthins. Betalains are widely used as natural red food colorant as well as antioxidant potentials. Several methods have been published for the determination of betalain in fruits of Opuntia species. The purpose of the current review is to provide a systematic survey of the analytical techniques for the determination of betalain from fruits of Opuntia species.

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References

  • Bilyk A (1979) Extractive fractionation of betalaines. J Food Sci 44:1249–1251

    Article  CAS  Google Scholar 

  • Bilyk A (1981) Thin layer chromatographic separation of beet pigments. J Food Sci 46:298–299

    Article  CAS  Google Scholar 

  • Cai Y, Corke H (1999) Amaranthus betacyanin pigments applied in model food systems. J Food Sci 64:869–873

    Article  CAS  Google Scholar 

  • Cai YZ, Sun Mei, Corke Harold (2005) Characterization and application of betalain pigments from plants of the Amaranthaceae. Trends Food Sci Technol 16:370–376

    Article  CAS  Google Scholar 

  • Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol 70:1–9

    Article  CAS  Google Scholar 

  • Chethana S, Nayak CA, Raghavarao KSMS (2007) Aqueous two phase extraction for purification and concentration of betalains. J Food Eng 81:679–687

    Article  CAS  Google Scholar 

  • Delgado-Vargas F, Jimenez R, Paredes-Lopes O (2000) Natural Pigments: carotenoids, anthocyanins, and betalains—characteristics, biosynthesis, processing, and stability. Crit Rev Food Sci 40(3):173–289

    Article  CAS  Google Scholar 

  • Fernández-López JA, Almela L (2001) Application of high-performance liquid chromatography to the characterization of the betalain pigments in prickly pear fruits. J Chromatogr A 913:415–420

    Article  PubMed  Google Scholar 

  • Fernández-López JA, Castellar R, Obon JM, Almela L (2002) Screening and mass-spectral confirmation of betalains in cactus pears. Chromatographia 56:591–595

    Article  Google Scholar 

  • Harborne JB (2007) Phytochemical methods—a guide to modern techniques of plant analysis, Springer international edition

  • Nilsson T (1970) Studies into the pigments in beetroot. Lantbrukshogskölans Annaler 36:179–219

    CAS  Google Scholar 

  • Piga A (2004) Cactus pear: a fruit of nutraceutical and functional importance. J Prof Assoc Cactus Dev 6:9–22

    Google Scholar 

  • Schliemann W et al (1996) Betacyanin from plants and cell cultures of Phytolacca americana. Phytochemistry 40(4):1039–1046

    Article  Google Scholar 

  • Schliemann W et al (2000) Betalains from Christmas cactus. Phytochemestry 54:419–426

    Article  Google Scholar 

  • Schliemann W et al (2001) Betalains of Celosia argentea. Phytochemistry 58:159–165

    Article  PubMed  CAS  Google Scholar 

  • Stintzing FC, Carle R (2004) Functional properties of anthocyanins and betalains in plants, food, and in human nutrition. Trends Food Sci Technol 15:19–38

    Article  CAS  Google Scholar 

  • Stintzing FC, Carle R (2007) Betalains-emerging prospects for food scientists. Trends Food Sci Technol 18:514–525

    Article  CAS  Google Scholar 

  • Stintzing FC, Schieber A, Carle R (2003) Evaluation of colour properties and chemical quality parameters of cactus juices. Eur Food Res Technol 216:303–311

    CAS  Google Scholar 

  • Stintzing FC, Conrad J, Klaiber I, Beifuss U, Carle R (2004) Structural investigations on betacyanin pigments by LC NMR and 2D NMR spectroscopy. Phytochemistry 65:415–422

    Article  PubMed  CAS  Google Scholar 

  • Stintzing FC, Moßhammer MR, Carle R (2005) Development of a process for the production of a betalain-based colouring foodstuff from cactus pear. Innov Food Sci Emerg 6:221–231

    Article  Google Scholar 

  • Stintzing FC, Moßhammer MR, Carle R (2006a) Evaluation of different methods for the production of juice concentrates and fruit powders from cactus pear. Innov Food Sci Emerg 7:275–287

    Article  Google Scholar 

  • Stintzing FC, Kugler F, Carle R, Conrad J (2006b) First 13CNMR assignments of betaxanthins. Helv Chim Acta 89:1008–1016

    Article  CAS  Google Scholar 

  • Strack D, Vogt T, Schliemann W (2003) Recent advances in betalain research. Phytochemistry 62:247–269

    Article  PubMed  CAS  Google Scholar 

  • Trezzini GF, Zr JP (1991) Two betalains from Portucala grandiflora. Phytochemistry 30:1897–1899

    Article  CAS  Google Scholar 

  • Viloria-Matos A, Moreno-Alvarez MJ, Hidalgo-Baez D (2001) Isolation and Identification of Betacyanin from fruits of Opuntia boldinghii Br. et R. by HPTLC. Cienc Tecnol Aliment 3(3):140–143

    Article  CAS  Google Scholar 

  • Wybraniec S (2006) Effect of tetraalkylammonium salts on retention of betacyanins and decarboxylated betacyanins in ion-pair reversed-phase high-performance liquid chromatography. J Chromatogr A 1127:70–75

    Article  PubMed  CAS  Google Scholar 

  • Wybraniec S (2008) Chromatographic investigation on acyl migration in betacyanins and their decarboxylated derivatives. J Chromatogr B 861:40–47

    Article  CAS  Google Scholar 

  • Wybraniec S et al (2001) Betacyanin from vine cactus Hylocereus polyrhizus. Phytochemistry 58:1209–1212

    Article  PubMed  CAS  Google Scholar 

  • Wybraniec S, Nowark-Wydra B, Mizrahi Y (2006) 1H and 13C NMR spectroscopic structural elucidation of new decarboxylated betacyanins. Tetrahedron Lett 47:1725–1728

    Article  CAS  Google Scholar 

  • Yahia EM, Castellanos-Santiago E (2008) Identification and quantification of betalains from the fruits of 10 Mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry. J Agric Food Chem 56:5758–5764

    Article  PubMed  Google Scholar 

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Correspondence to Sanjay P. Chauhan.

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Chauhan, S.P., Sheth, N.R., Rathod, I.S. et al. Analysis of betalains from fruits of Opuntia species. Phytochem Rev 12, 35–45 (2013). https://doi.org/10.1007/s11101-012-9248-2

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  • DOI: https://doi.org/10.1007/s11101-012-9248-2

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