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Effects of hypo- and hypersalinity on photosynthetic performance of Sargassum fusiforme (Fucales, Heterokontophyta)

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Photosynthetica

Abstract

Photoprotection mechanisms protect photosynthetic organisms, especially under stress conditions, against photodamage that may inhibit photosynthesis. We investigated the effects of short-term immersion in hypo- and hypersalinity sea water on the photosynthesis and xanthophyll cycle in Sargassum fusiforme (Harvey) Setchell. The results indicated that under moderate light [110 μmol(photon) m−2 s−1], the effective quantum yield of PSII was not reduced in S. fusiforme fronds after 1 h in hyposalinity conditions, even in fresh water, but it was significantly affected by extreme hypersalinity treatment (90‰ sea water). Under high light [HL, 800 μmol(photon) m−2 s−1], photoprotective mechanisms operated efficiently in fronds immersed in fresh water as indicated by high reversible nonphotochemical quenching of chlorophyll fluorescence (NPQ) and de-epoxidation state; the quantum yield of PSII recovered during the subsequent relaxation period. In contrast, fronds immersed in 90‰ sea water did not withstand HL, barely developed reversible NPQ, and accumulated little antheraxanthin and zeaxanthin during HL, while recovery of the quantum yield of PSII was severely inhibited during the subsequent relaxation period. The data provided concrete evidence supporting the short-term tolerance of S. fusiforme to immersion in fresh water compared to hypersalinity conditions. The potential practical implications of these results were also discussed.

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Abbreviations

AL:

actinic light

Ax:

antheraxanthin

Chl:

chlorophyll

DEPS:

de-epoxidation state

DTT:

dithiothreitol

F:

fluorescence under illumination

F0 :

minimal fluorescence yield of the dark-adapted state

Fm :

maximal fluorescence yield of the dark-adapted state

Fm′:

maximal fluorescence yield of the light-adapted state

Fv/Fm :

maximal quantum yield of PSII photochemistry

HL:

high light

Ik :

light saturation coefficient

NPQ:

nonphotochemical quenching

rETR:

relative electron transport rate

rETRmax :

maximal relative electron transport rate

RLC:

rapid light curve

ROS:

reactive oxygen species

SP:

saturation pulse

Vx:

violaxanthin

Zx:

zeaxanthin

FPSII :

effective quantum yield of PSII

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China

    X. J. Xie, X. L. Wang, L. W. He, W. H. Gu, S. Gao & G. C. Wang

  2. Zhejiang Provincial Key Lab for Water Environment and Marine Biological Resources Protection, College of Life and Environment Science, Wenzhou University, 325035, Wenzhou, China

    L. D. Lin, X. F. Yan & M. J. Wu

  3. Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 266071, Qingdao, China

    X. J. Xie, X. L. Wang, L. W. He, W. H. Gu, S. Gao & G. C. Wang

  4. University of Chinese Academy of Sciences, 100039, Beijing, China

    X. L. Wang

  5. Tianjin Key Laboratory of Marine Sources and Chemistry, College of Marine Science & Engineering, Tianjin University of Science & Technology, 300457, Tianjin, China

    G. H. Pan

Authors
  1. X. J. Xie
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  2. X. L. Wang
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  3. L. D. Lin
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  4. L. W. He
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  5. W. H. Gu
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  8. G. H. Pan
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  9. M. J. Wu
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  10. G. C. Wang
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Corresponding authors

Correspondence to M. J. Wu or G. C. Wang.

Additional information

Acknowledgements: This work was supported by Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, the nonprofit research project for the State Oceanic Administration (201105008-2); supported project (2012BAC07B03); China Strategic Leading Special Science and Technology Academy (XDA11020404); and 863 Project (2012AA100811-5).

Author contributions: G.C. Wang, M.J. Wu, and X.J. Xie conceived and designed the research. X.J. Xie, X.L. Wang, L.D. Lin, and L.W. He conducted the experiments. W.H. Gu, S. Gao, X.F. Yan, and G.H. Pan analyzed data. X.J. Xie and G.C. Wang wrote the manuscript.

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Xie, X.J., Wang, X.L., Lin, L.D. et al. Effects of hypo- and hypersalinity on photosynthetic performance of Sargassum fusiforme (Fucales, Heterokontophyta). Photosynthetica 54, 210–218 (2016). https://doi.org/10.1007/s11099-015-0169-3

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  • DOI: https://doi.org/10.1007/s11099-015-0169-3

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