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Rice varietal differences in responses of stomatal gas exchange to supplemental nitrogen application

  • Original Papers
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Photosynthetica

Abstract

In the field, supplemental application of N fertilizer to rice (Oryza sativa) shortly before the beginning of heading stage increases leaf N content and enhances photosynthesis during the grain-filling period. In search of varietal differences in leaf gas exchange in response to supplemental N application, we examined 13 rice varieties grown in the field during two successive years. The varieties included japonica and indica varieties, both of which are widely grown in Japan. The response to supplemental N application could not be separated clearly between variety groups; some of the japonica varieties, but none of the indica varieties, exhibited significant increase in stomatal conductance (g s) after supplemental N application. Supplemental N was more effective to increase stomatal aperture in the varieties with inherently lower g s. Varieties that showed greater response of g s to supplemental N application might be able to adjust their stomatal aperture with appropriate N control. Although the internal-to-ambient CO2 mole fraction ratio and the leaf carbon isotopic composition (δ13C) differed among varieties as a result of variations in stomatal aperture and the CO2 requirement of mesophyll, supplemental N application barely influenced these parameters, because it only moderately affected stomatal aperture. Since δ13C tended to increase with increasing number of days from transplantation to heading stage in japonica varieties, δ13C values were more sensitive to differences in growth rate between years than to N application.

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Abbreviations

C i/C a :

ratio of intercellular to ambient CO2 mole fraction

g s :

stomatal conductance

P N :

net photosynthetic rate

δ13C:

carbon isotope composition

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Authors and Affiliations

  1. National Agriculture and Food Research Organization, Hokkaido Agricultural Research Center, Shinsei-Minami, Memuro, Kasai, Hokkaido, 082-0081, Japan

    S. Shimoda

  2. National Agriculture and Food Research Organization, Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    A. Maruyama

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  1. S. Shimoda
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  2. A. Maruyama
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Correspondence to S. Shimoda.

Additional information

Acknowledgements: I thank Kenji Nagata and Hidekazu Kobayashi (NARO Western Region Agricultural Research Center; NARO/WARC) for their help with the experiments, Dairo Yamashita, Tastuya Okada, and Koji Yamazaki (NARO/WARC) for their help with crop management, and Tomoko Ogawa and Yoshinobu Fujimoto (NARO/WARC) for their assistance with the experiments.

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Shimoda, S., Maruyama, A. Rice varietal differences in responses of stomatal gas exchange to supplemental nitrogen application. Photosynthetica 52, 397–403 (2014). https://doi.org/10.1007/s11099-014-0043-8

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  • DOI: https://doi.org/10.1007/s11099-014-0043-8

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