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A newly developed SGB buffer greatly enhances energy transfer efficiency from phycobilisomes to photosystem II in cyanobacteria in vitro

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Photosynthetica

Abstract

The transfer of light energy from phycobilisomes (PBS) to photosystem II (PSII) reaction centers is vital for photosynthesis in cyanobacteria and red algae. To investigate the relationship between PBS and PSII and to optimize the energy transfer efficiency from PBS to PSII, isolation of the PBS-PSII supercomplex is necessary. SPC (sucrose/phosphate/citrate) is a conventional buffer for isolating PBS-PSII supercomplex in cyanobacteria. However, the energy transfer occurring in the supercomplex is poor. Here, we developed a new buffer named SGB by adding 1M glycinebetaine and additional sucrose to SPC buffer. Compared to SPC, the newly developed SGB buffer greatly enhanced the associated populations of PBS with thylakoid membranes and PSII and further improved the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro. Therefore, we conclude that SGB is an excellent buffer for isolating the PBS-PSII supercomplex and for enhancing the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro.

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Abbreviations

APC:

allophycocyanin

Chl:

chlorophyll

GB:

glycinebetaine

NQ:

1,4-naphthoquinone

PBS:

phycobilisome

PC:

phycocyanin

PSII:

photosystem II

SGB:

sucrose/phosphate/citrate/glycinebetaine

SPC:

sucrose/phosphate/citrate

Suc:

sucrose

Synechocystis 6803:

Synechocystis sp. strain PCC 6803

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Authors and Affiliations

  1. School of Life Science, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China

    L. P. Chen, Q. X. Wang & W. M. Ma

  2. College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai, 200234, China

    L. P. Chen & Q. X. Wang

Authors
  1. L. P. Chen
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  2. Q. X. Wang
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  3. W. M. Ma
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Corresponding authors

Correspondence to Q. X. Wang or W. M. Ma.

Additional information

Acknowledgements: This work was partially supported by Key Project of Science and Technology Commission of Shanghai (No. 09160500400), Key Project of Chinese Ministry of Education (No. 209045), National Basic Research Program of China (No. 2009CB118500), and Shanghai Municipal Education Commission (Nos. J50401, 11YZ89, and 12ZZ132).

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Chen, L.P., Wang, Q.X. & Ma, W.M. A newly developed SGB buffer greatly enhances energy transfer efficiency from phycobilisomes to photosystem II in cyanobacteria in vitro . Photosynthetica 51, 215–220 (2013). https://doi.org/10.1007/s11099-013-0018-1

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  • DOI: https://doi.org/10.1007/s11099-013-0018-1

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