Preparation of Drug-Loaded PLGA-PEG Nanoparticles by Membrane-Assisted Nanoprecipitation

Research Paper

DOI: 10.1007/s11095-017-2146-y

Cite this article as:
Albisa, A., Piacentini, E., Sebastian, V. et al. Pharm Res (2017). doi:10.1007/s11095-017-2146-y
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Abstract

Purpose

The aim of this work is to develop a scalable continuous system suitable for the formulation of polymeric nanoparticles using membrane-assisted nanoprecipitation. One of the hurdles to overcome in the use of nanostructured materials as drug delivery vectors is their availability at industrial scale. Innovation in process technology is required to translate laboratory production into mass production while preserving their desired nanoscale characteristics.

Methods

Membrane-assisted nanoprecipitation has been used for the production of Poly[(D,L lactide-co-glycolide)-co-poly ethylene glycol] diblock) (PLGA-PEG) nanoparticles using a pulsed back-and-forward flow arrangement. Tubular Shirasu porous glass membranes (SPG) with pore diameters of 1 and 0.2 μm were used to control the mixing process during the nanoprecipitation reaction.

Results

The size of the resulting PLGA-PEG nanoparticles could be readily tuned in the range from 250 to 400 nm with high homogeneity (PDI lower than 0.2) by controlling the dispersed phase volume/continuous phase volume ratio. Dexamethasone was successfully encapsulated in a continuous process, achieving an encapsulation efficiency and drug loading efficiency of 50% and 5%, respectively. The dexamethasone was released from the nanoparticles following Fickian kinetics.

Conclusions

The method allowed to produce polymeric nanoparticles for drug delivery with a high productivity, reproducibility and easy scalability.

Key Words

dexamethasone membrane emulsification nanoparticles nanoprecipitation PLGA-peg 

Abbreviations

CP

Continuous phase

DEX

Dexamethasone

DLE

Drug loading efficiency

DP

Dispersed phase

EE

Encapsulation efficiency

MANA

Membrane-assisted nanoprecipitacion

NPs

Nanoparticles

NSBTR

Nanoprecipitation in a stirred batch-type reactor

PDI

Polydispersity index

PEG

Poly ethylene glycol

PGA

Glycolic acids

PLA

Lactic acid

PLGA-PEG

Poly[(D,L lactide-co-glycolide)-co-poly ethylene glycol] diblock

SEM

Scanning Electron Microscopy

Supplementary material

11095_2017_2146_MOESM1_ESM.docx (897 kb)
ESM 1(DOCX 896 kb)

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Chemical & Environmental Engineering & Nanoscience Institute of Aragon (INA),University of Zaragoza,ZaragozaSpain
  2. 2.Department of Environmental and Chemical EngineeringUniversity of Calabria (DIATIC-UNICAL),RendeItaly
  3. 3.Institute on Membrane Technology,National Research Council ITM-CNRRendeItaly
  4. 4.CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Centro de Investigación Biomédica en RedMadridSpain

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