Abstract
Purpose
This work describes the synthesis of block co-polymeric micelles, methoxy-poly(ethylene glycol)-poly(D,L-lactide) (mPEG-PLA) to encapsulate Curcumin (CUR), thereby improving the dispersibility and chemical stability of curcumin, prolonging its cellular uptake and enhancing its bioavailability.
Methods
CUR-mPEG-PLA micelles, was prepared using the thin-film hydration method and evaluated in vitro. The preparation process was optimized with a central composite design (CCD). Micelles were characterized by size, transmission electron microscopy, loading capacity, and critical micelle concentration (CMC). The cytotoxicity of CUR-mPEG-PLA micelles was investigated against murine melanoma cells, B16F10 and human breast cancer cells, MDA-MB-231.
Results
The average size of the CUR-mPEG-PLA micelles was 110 ± 5 nm with polydispersity index in the range of 0.15–0.31, and the encapsulating efficiency for CUR was 91.89 ± 1.2, and 11.06 ± 0.8% for drug-loading. Sustained release of CUR from micelles was observed with 9.73% CUR release from micelles compared to 64.24% release of free curcumin in first 6 h under sink condition. The CUR-mPEG-PLA was efficiently taken up by the cancer cells, B16F10 and MDA-MB-231. Following 24 h incubation, CUR-mPEG-PLA induced higher cytotoxicity compared to free CUR in MDA-MB-231 cell lines indicating exposure of higher dose of free CUR to cells lead to up-regulation of drug efflux mechanisms leading to decreased cell death in case of free CUR administration.
Conclusion
Our results indicate that the proposed micellar system has the potential to serve as an efficient carrier for CUR by effectively solubilizing, stabilizing and delivering the drug in a controlled manner to the cancer cells.
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Abbreviations
- B16F10:
-
Murine melanoma cells
- CCD:
-
Central composite design
- CMC:
-
Critical micelle concentration
- CUR:
-
Curcumin
- DMEM:
-
Dulbecco’s modified Eagle’s media
- DSC:
-
Differential scanning calorimetry
- EPR:
-
Enhanced Permeability and Retention
- FTIR:
-
Fourier transform infrared spectroscopy
- HepG2:
-
Human hepatocellular carcinoma cells
- MDA-MB-231:
-
Human breast cancer cells
- mPEG:
-
Methoxy-poly(ethylene glycol)
- mPEG-PLA:
-
Methoxy-poly(ethylene glycol)-poly(D,L-Lactide)
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide
- MWCO:
-
Molecular weight cut-off
- NMR:
-
Nuclear magnetic resonance
- PEG:
-
Poly (ethylene glycol)
- PLA:
-
Poly(lactic acid)
- RPMI-1640:
-
Roswell Park Memorial Institute 1640 media
- TEM:
-
Transmission electron microscope
- THF:
-
Tetrahydrofuran
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Acknowledgments and Disclosures
The work was supported in part by the grants provided by the Department of Science and Technology (CS-269/2013), and Department of Biotechnology (BT/Bio-CARe/07/10003/2013-14), Government of India to Swati Biswas. Preeti Kumari gratefully acknowledges INSPIRE (IF130703), Department of Science and Technology (DST), Ministry of Science and Technology, Government of India for awarding her with the Junior Research Fellowship. The authors declare no conflict of interest.
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Kumari, P., Swami, M.O., Nadipalli, S.K. et al. Curcumin Delivery by Poly(Lactide)-Based Co-Polymeric Micelles: An In Vitro Anticancer Study. Pharm Res 33, 826–841 (2016). https://doi.org/10.1007/s11095-015-1830-z
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DOI: https://doi.org/10.1007/s11095-015-1830-z