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Activation of Antigen-Specific T Cell-Responses by Mannan-Decorated PLGA Nanoparticles

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ABSTRACT

Purpose

Mannosylation of vaccines is a promising strategy to selectively target vaccine antigens to the mannose receptor expressed on dendritic cells (DCs). The purpose of this study was to investigate the effect of mannan (MN) chemically conjugated to poly(D, L-lactide-co-glycolic acid) (PLGA) nanoparticles (NPs) on antigen-specific T-cell responses elicited by a model antigen (ovalbumin, OVA) loaded in PLGA-NPs.

Methods

In vitro T-cell proliferation assay was done to assess the ability of DCs treated with OVA-NPs (±MN decoration) to induce antigen-specific T-cell activation. The efficacy of this vaccination strategy was further evaluated in vivo, where T-cell proliferation was performed to evaluate activation of T-cell responses in lymph nodes and spleens isolated from the vaccinated mice.

Results

Our results demonstrate that MN-decorated antigen-loaded PLGA-NPs simultaneously enhanced antigen-specific CD4+ and CD8+ T-cell responses compared to non-decorated NPs.

Conclusions

MN decoration of PLGA-NPs is a promising strategy for enhancing antigen-specific T-cell responses.

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ACKNOWLEDGMENTS

The authors would like to thank Elaine Moase for proof-reading the manuscript. The authors would like to acknowledge financial support by research grant from Natural Sciences and Engineering Council of Canada (STPGP 336987). This manuscript is dedicated to Prof. Dr. John Samuel, our mentor, who passed away in April 2007 and was an expert in the field of cancer immunotherapy. The authors report no conflict of interest.

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Authors and Affiliations

  1. Faculty of Pharmacy and Pharmaceutical Sciences 3133 Dentistry/Pharmacy Centre, University of Alberta, Edmonton, Alberta, Canada

    Samar Hamdy, Anooshirvan Shayeganpour, John Samuel & Afsaneh Lavasanifar

  2. Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Azita Haddadi

  3. Faculty of Engineering Department of Chemical and Material Engineering, University of Alberta, Edmonton, Alberta, Canada

    Afsaneh Lavasanifar

  4. Faculty of Pharmacy and Pharmaceutical Sciences 4119 Dent/Pharm Centre, University of Alberta, Edmonton, Alberta, T6G2N8, Canada

    Afsaneh Lavasanifar

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  1. Samar Hamdy
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  2. Azita Haddadi
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  4. John Samuel
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  5. Afsaneh Lavasanifar
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Correspondence to Azita Haddadi or Afsaneh Lavasanifar.

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Hamdy, S., Haddadi, A., Shayeganpour, A. et al. Activation of Antigen-Specific T Cell-Responses by Mannan-Decorated PLGA Nanoparticles. Pharm Res 28, 2288–2301 (2011). https://doi.org/10.1007/s11095-011-0459-9

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  • DOI: https://doi.org/10.1007/s11095-011-0459-9

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