Origins of Life and Evolution of Biospheres

, Volume 42, Issue 6, pp 533–541

The Use of Ascorbate as an Oxidation Inhibitor in Prebiotic Amino Acid Synthesis: A Cautionary Note

Authors

    • Department of Chemistry and BiotechnologyYokohama National University
    • Department of Complexity Science and Engineering, Graduate School of Frontier SciencesThe University of Tokyo
  • Midori Eto
    • Department of Chemistry and BiotechnologyYokohama National University
  • Yukinori Kawamoto
    • Department of Chemistry and BiotechnologyYokohama National University
  • Hironari Kurihara
    • Department of Chemistry and BiotechnologyYokohama National University
  • Takeo Kaneko
    • Department of Chemistry and BiotechnologyYokohama National University
  • Yumiko Obayashi
    • Department of Chemistry and BiotechnologyYokohama National University
  • Kensei Kobayashi
    • Department of Chemistry and BiotechnologyYokohama National University
Prebiotic Chemistry

DOI: 10.1007/s11084-012-9296-z

Cite this article as:
Kuwahara, H., Eto, M., Kawamoto, Y. et al. Orig Life Evol Biosph (2012) 42: 533. doi:10.1007/s11084-012-9296-z

Abstract

It is generally thought that the terrestrial atmosphere at the time of the origin of life was CO2-rich and that organic compounds such as amino acids would not have been efficiently formed abiotically under such conditions. It has been pointed out, however, that the previously reported low yields of amino acids may have been partially due to oxidation by nitrite/nitrate during acid hydrolysis. Specifically, the yield of amino acids was found to have increased significantly (by a factor of several hundred) after acid hydrolysis with ascorbic acid as an oxidation inhibitor. However, it has not been shown that CO2 was the carbon source for the formation of the amino acids detected after acid hydrolysis with ascorbic acid. We therefore reinvestigated the prebiotic synthesis of amino acids in a CO2-rich atmosphere using an isotope labeling experiment. Herein, we report that ascorbic acid does not behave as an appropriate oxidation inhibitor, because it contributes amino acid contaminants as a consequence of its reactions with the nitrogen containing species and formic acid produced during the spark discharge experiment. Thus, amino acids are not efficiently formed from a CO2-rich atmosphere under the conditions studied.

Keywords

Amino acidPrebiotic synthesisCO2-rich atmosphereSpark discharge

Copyright information

© Springer Science+Business Media B.V. 2012