Abstract
Glycogen synthase kinase 3β (GSK-3β) is a key downstream protein in the PI3K/Akt pathway. Phosphorylation of serine 9 of GSK-3β (GSK-3β activity inhibition) promotes cell survival. In this study, we examined changes in expressions of GSK-3β and phosphorylation of GSK-3β (p-GSK-3β) in the gerbil hippocampal CA1 area after 5 min of transient cerebral ischemia. GSK-3β immunoreactivity in the CA1 area was increased in pyramidal cells at 6 h after ischemia–reperfusion. It was decreased in CA1 pyramidal cells from 12 h after ischemia–reperfusion, and hardly detected in the CA1 pyramidal cells at 5 days after ischemia–reperfusion. p-GSK-3β immunoreactivity was slightly decreased in CA1 pyramidal cells at 6 and 12 h after ischemia–reperfusion. It was significantly increased in these cells at 1 and 2 days after ischemia–reperfusion. Five days after ischemia–reperfusion, p-GSK-3β immunoreactivity was hardly found in CA1 pyramidal cells. However, p-GSK-3β immunoreactivity was strongly expressed in astrocytes primarily distributed in strata oriens and radiatum. In conclusion, GSK-3β and p-GSK-3β were significantly changed in pyramidal cells and/or astrocytes in the gerbil hippocampal CA1 area following 5 min of transient cerebral ischemia. This finding indicates that GSK-3β and p-GSK-3β are closely related to delayed neuronal death.
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Acknowledgements
This research was supported by the Bio-Synergy Research Project (NRF-2015M3A9C4076322) of the Ministry of Science, ICT and Future Planning through the National Research Foundation, and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Science, ICT and Future Planning (NRF-2014R1A1A3051721).
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Bai Hui Chen and Ji Hyeon Ahn they have contributed equally to this work.
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Chen, B.H., Ahn, J.H., Park, J.H. et al. Transient Cerebral Ischemia Alters GSK-3β and p-GSK-3β Immunoreactivity in Pyramidal Neurons and Induces p-GSK-3β Expression in Astrocytes in the Gerbil Hippocampal CA1 Area. Neurochem Res 42, 2305–2313 (2017). https://doi.org/10.1007/s11064-017-2245-5
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DOI: https://doi.org/10.1007/s11064-017-2245-5