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Curcumin Attenuated Bupivacaine-Induced Neurotoxicity in SH-SY5Y Cells Via Activation of the Akt Signaling Pathway

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Abstract

Bupivacaine is widely used for regional anesthesia, spinal anesthesia, and pain management. However, bupivacaine could cause neuronal injury. Curcumin, a low molecular weight polyphenol, has a variety of bioactivities and may exert neuroprotective effects against damage induced by some stimuli. In the present study, we tested whether curcumin could attenuate bupivacaine-induced neurotoxicity in SH-SY5Y cells. Cell injury was evaluated by examining cell viability, mitochondrial damage and apoptosis. We also investigated the levels of activation of the Akt signaling pathway and the effect of Akt inhibition by triciribine on cell injury following bupivacaine and curcumin treatment. Our findings showed that the bupivacaine treatment could induce neurotoxicity. Pretreatment of the SH-SY5Y cells with curcumin significantly attenuated bupivacaine-induced neurotoxicity. Interestingly, the curcumin treatment increased the levels of Akt phosphorylation. More significantly, the pharmacological inhibition of Akt abolished the cytoprotective effect of curcumin against bupivacaine-induced cell injury. Our data suggest that pretreating SH-SY5Y cells with curcumin provides a protective effect on bupivacaine-induced neuronal injury via activation of the Akt signaling pathway.

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Acknowledgments

This study was supported by Grants from the National Natural Science Foundation of China (Nos. 81471272, 81271390). None of the authors have financial relationships with biotechnology manufacturers, pharmaceutical companies, or other commercial entities with an interest in the subject matter or materials discussed in the manuscripts.

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Authors and Affiliations

  1. Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, 253 Industrial Road, Guangzhou, 510282, Guangdong Province, China

    You-Ling Fan, Heng-Chang Li, Wei Zhao & Shi-Yuan Xu

  2. Department of Anesthesiology, Central Hospital of Panyu District, Guangzhou, 511400, Guangdong Province, China

    You-Ling Fan, Hui-Hua Peng, Fang Huang & Wei-Hang Jiang

  3. Department of Anesthesiology, Guangzhou First People’s Hospital, Guangzhou, 510180, Guangdong Province, China

    Heng-Chang Li

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  1. You-Ling Fan
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Correspondence to Shi-Yuan Xu.

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Fan, YL., Li, HC., Zhao, W. et al. Curcumin Attenuated Bupivacaine-Induced Neurotoxicity in SH-SY5Y Cells Via Activation of the Akt Signaling Pathway. Neurochem Res 41, 2425–2432 (2016). https://doi.org/10.1007/s11064-016-1955-4

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  • DOI: https://doi.org/10.1007/s11064-016-1955-4

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