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Progesterone Attenuates Aquaporin-4 Expression in an Astrocyte Model of Ischemia/Reperfusion

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Abstract

Previous studies have suggested that progesterone may be involved in neuroprotection by preventing brain edema. In this study, we assessed the effects of progesterone on aquaporin-4 (AQP4) expression in an ischemia/reperfusion model of cultured rat astrocytes, and further explored the possible role of the protein kinase C (PKC) pathway in this course. We evaluate primary culture astrocytes exposed to 4 h oxygen–glucose deprivation (OGD) followed by 24 h reperfusion (OGD4h/R24h) as a means of simulating cortex ischemia and reperfusion, and test the effect of progesterone on AQP4 expression in response to OGD4h/R24h. Besides, the cell viability was assessed by MTT reduction and lactate dehydrogenase release assay, accompanied by cell morphology survey. At a concentration of 1 and 2 μM, progesterone significantly attenuated AQP4 at the level of both protein and mRNA and ameliorated the cell viability of astrocytes from OGD/reperfusion injury. Moreover, this effect was blocked by the PKC inhibitor Ro31-8220, which was employed before the OGD. These results indicate that progesterone exerts the protective effects and attenuates AQP4 expression in an astrocyte model of ischemia/reperfusion depending on the PKC signal pathway.

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Acknowledgments

We thank professor Wenyan Niu for her invaluable assistance with the astrocyte model of ischemia/reperfusion. This study was supported by the National Natural Science Foundation of China (no. 81200957) and the key research project fund of Tianjin Medical University (no. 052-200010).

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Authors and Affiliations

  1. Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Tianjin, 300070, China

    Lu He, Xiaoyang Zhang, Xianfeng Wei & Yunsheng Li

  2. Department of Otolaryngology, Tianjin First Center Hospital, 24 Rehabilitation Road, Tianjin, 300192, China

    Xianfeng Wei

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  1. Lu He
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Correspondence to Yunsheng Li.

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He, L., Zhang, X., Wei, X. et al. Progesterone Attenuates Aquaporin-4 Expression in an Astrocyte Model of Ischemia/Reperfusion. Neurochem Res 39, 2251–2261 (2014). https://doi.org/10.1007/s11064-014-1427-7

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